Novel transcribed regions in the human genome.
Novel transcribed regions in the human genome.
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人类基因组中的新转录区域。
DOI：
10.1101/sqb.2006.71.054
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发表时间：
2006
期刊：
影响因子：
--
通讯作者：
Snyder,M
中科院分区：
文献类型：
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作者：
Rozowsky,J;Wu,J;Lian,Z;Nagalakshmi,U;Korbel,JO;Kapranov,P;Zheng,D;Dyke,S;Newburger,P;Miller,P;Gingeras,TR;Weissman,S;Gerstein,M;Snyder,M
我们已经使用基因组平铺阵列来识别整个人类基因组中的转录区域。从NB 4细胞、维甲酸（RA）处理的NB 4细胞、12-O-十四烷酰佛波醇-13乙酸酯（TPA）处理的NB 4细胞、中性粒细胞和胎盘等5种细胞/组织中分离的RNA的ENCODE区域的定位结果分析显示了大量新的转录区域。有趣的是，中性粒细胞在几个内含子区域表现出大量新的表达。比较不同刺激物处理的NB 4细胞与未处理细胞的杂交结果，发现细胞分化后有许多新的区域表达。其中一个区域是Hox基因座，它包含了大量在多种细胞类型中表达的新区域。对新转录区的三核苷酸组成的分析表明，它与已知外显子的三核苷酸组成相似。这些结果表明，许多新的转录区域可能具有功能作用。
We have used genomic tiling arrays to identify transcribed regions throughout the human genome. Analysis of the mappingresults of RNA isolated from five cell/tissue types, NB4 cells, NB4 cells treated with retinoic acid (RA), NB4 cells treatedwith 12-O-tetradecanoylphorbol-13 acetate (TPA), neutrophils, and placenta, throughout the ENCODE region reveals alarge number of novel transcribed regions. Interestingly, neutrophils exhibit a great deal of novel expression in severalintronic regions. Comparison of the hybridization results of NB4 cells treated with different stimuli relative to untreated cellsreveals that many new regions are expressed upon cell differentiation. One such region is the Hox locus, which contains alarge number of novel regions expressed in a number of cell types. Analysis of the trinucleotide composition of the noveltranscribed regions reveals that it is similar to that of known exons. These results suggest that many of the novel transcribedregions may have a functional role.