Phylogeny of the Venezuelan arenaviruses
Phylogeny of the Venezuelan arenaviruses
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DOI：
10.1016/j.virusres.2004.01.032
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发表时间：
2004-06-15
期刊：
影响因子：
5
通讯作者：
Fulhorst, CF
中科院分区：
文献类型：
--
作者：
Cajimat, MNB;Fulhorst, CF
瓜纳里托病毒（委内瑞拉出血热的病原体，VHF）和皮尔塔尔病毒在委内瑞拉出血热流行的委内瑞拉地区共存。本研究的目的是扩展我们对这两种沙粒病毒之间进化关系的认识。我们确定，瓜纳里托病毒和皮尔塔尔病毒的大基因组片段与其他南美沙粒病毒的大基因组片段大小相似，结构组织相同。例如，瓜纳里托病毒和皮尔塔尔病毒的Z蛋白和RNA依赖性RNA聚合酶在极性相反的非重叠开放阅读框中编码。Z蛋白基因核苷酸序列和RNA依赖性RNA聚合酶基因核苷酸序列的系统发育分析将Pirital病毒与Pichinde病毒（一种南美洲沙粒病毒，与Pirital病毒一样，似乎对人类没有致病性）分组，并将Pirital-Pichinde谱系与Guanarito病毒和已知在南美洲引起出血热的其他三种沙粒病毒代表的谱系置于姐妹关系中。这些结果与沙粒病毒小基因组片段的遗传学研究结果一致。因此，瓜纳里托病毒和皮尔塔尔病毒之间通过重组或重配进行的基因组元件交换可能不会导致委内瑞拉出血热的出现。(C)2004 Elsevier B. V.保留所有权利。
Guanarito virus (the etiologic agent of Venezuelan hemorrhagic fever, VHF) and Pirital virus coexist in the region of Venezuela in which Venezuelan hemorrhagic fever is endemic. The purpose of this study was to extend our knowledge of the evolutionary relationship between these two arenaviruses. We determined that the large genomic segments of Guanarito virus and Pirital virus are similar in size and identical in structural organization to the large genomic segments of other South American arenaviruses. For example, the Z proteins and RNA-dependent RNA polymerases of Guanarito virus and Pirital virus are encoded in nonoverlapping open reading frames of opposite polarities. Phylogenetic analyses of Z protein gene nucleotide sequences and RNA-dependent RNA polymerase gene nucleotide sequences grouped Pirital virus with Pichinde virus (a South American arenavirus which, like Pirital virus, does not appear to be pathogenic for humans) and placed the Pirital-Pichinde lineage in a sister relationship to a lineage represented by Guanarito virus and the three other arenaviruses known to cause hemorrhagic fever in South America. These results are concordant with the results of studies on the phylogeny of the arenavirus small genomic segment. Thus, the exchange of genomic elements between Guanarito virus and Pirital virus via recombination or reassortment likely did not contribute to the emergence of Venezuelan hemorrhagic fever. (C) 2004 Elsevier B.V. All rights reserved.