Aquaporin and aquaglyceroporin in silkworms, differently expressed in the hindgut and midgut of Bombyx mori
Aquaporin and aquaglyceroporin in silkworms, differently expressed in the hindgut and midgut of Bombyx mori
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DOI：
10.1111/j.1365-2583.2009.00871.x
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发表时间：
2009-06-01
影响因子：
2.6
通讯作者：
Azuma, M.
中科院分区：
文献类型：
--
作者：
Kataoka, N.;Miyake, S.;Azuma, M.
从家蚕幼虫中鉴定和鉴定了两个与其他昆虫的水通道蛋白(AQPs)相似的cDNA。从前丝腺克隆的第一个基因(AQP-Bom1)编码一个25900 Da的蛋白，与从几种流食性昆虫中分离到的昆虫的AQP相似。从中肠后段克隆的第二个基因(AQP-Bom2)编码27694Da的蛋白质。Northern印迹分析表明，AQP-Bom1mRNA(2.3kb)主要在后肠(结肠和直肠)表达，丝腺、中肠和马氏管中等或少量表达，而AQP-Bom2mRNA(1.3kb)主要在后中肠和马氏管表达。对显微注射这些水通道蛋白CRNA的非洲爪哇卵母细胞的功能分析表明，AQP-Bom1基因编码一个水特异的水通道蛋白，可能参与后肠的水回收功能，而AQP-Bom2基因编码一个水甘油通道蛋白，增加甘油和尿素的吸收。
Two cDNAs similar to aquaporins (AQPs) from other insect species were identified and characterized from the silkworm larva, Bombyx mori. The first cDNA (AQP-Bom1) cloned from the anterior silk gland encodes a 25 900 Da protein similar to insect AQPs isolated from several liquid-feeding insects. The second cDNA (AQP-Bom2) cloned from the posterior midgut encodes a 27 694 Da protein. Northern blot analysis has revealed that the AQP-Bom1 mRNA (2.3 kb) is expressed predominantly in the hindgut (colon and rectum), and moderately or minimally in the silk gland, midgut and Malpighian tubules, while the AQP-Bom2 mRNA (1.3 kb) is mainly expressed in the posterior midgut and Malpighian tubules. Functional analysis in Xenopus oocytes microinjected with the cRNA of these AQPs revealed that the AQP-Bom1 mRNA encodes a water-specific aquaporin, likely involved in the water retrieval function of the hindgut, while the AQP-Bom2 mRNA encodes an aquaglyceroporin, increasing glycerol and urea uptake.