Effect of changing temperature on anaerobic hydrogen production and microbial community composition in an open-mixed culture bioreactor
Effect of changing temperature on anaerobic hydrogen production and microbial community composition in an open-mixed culture bioreactor
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DOI：
10.1016/j.ijhydene.2010.07.070
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发表时间：
2010-10-01
影响因子：
7.2
通讯作者：
Puhakka, Jaakko A.
中科院分区：
文献类型：
--
作者：
Karadag, Dogan;Puhakka, Jaakko A.
研究了温度（37 ~ 65℃）对开放式混合培养生物反应器中葡萄糖产H-2的影响。采用聚合酶链反应-变性梯度凝胶电泳（PCR-DGGE）技术研究了微生物群落的动态变化。在45℃和60℃时H-2产量最高，分别为1.71和0.85 mol H-2/mol葡萄糖。在50℃和55℃条件下，H-2产率为0,37℃~ 45℃条件下，H-2产率为丁酸型发酵，60℃条件下，H-2产率为乙醇型发酵，37℃~ 45℃条件下，梭状芽孢杆菌种类占优势，50℃~ 55℃和60℃条件下，H-2产率分别为凝固芽孢杆菌和热厌氧杆菌。在b型凝血菌存在的情况下，代谢被导向乳酸生成。结果表明，混合培养有两个产H-2的最佳条件，微生物群落和代谢模式随温度的变化而迅速变化。(C) 2010 T. Nejat Veziroglu教授。Elsevier Ltd.出版。版权所有。
The temperature effect (37-65 degrees C) on H-2 production from glucose in an open-mixed culture bioreactor using an enrichment culture from a hot spring was studied. The dynamics of microbial communities was investigated by polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE). At 45 and 60 degrees C the H-2 production was the highest i.e. 1.71 and 0.85 mol H-2/mol glucose, respectively. No H-2 was produced at temperatures 50 and 55 degrees C. At 37-45 degrees C, H-2 production was produced by butyrate type fermentation while fermentation mechanism changed to ethanol type at 60 degrees C. Clostridium species were dominant at 37-45 degrees C while at 50-55 degrees C and 60 degrees C the culture was dominated by Bacillus coagulans and Thermoanaerobacterium, respectively. In the presence of B. Coagulans the metabolism was directed to lactate production. The results show that the mixed culture had two optima for H-2 production and that the microbial communities and metabolic patterns promptly changed according to changing temperatures. (C) 2010 Professor T. Nejat Veziroglu. Published by Elsevier Ltd. All rights reserved.