INCREASED LEVELS OF TRKB MESSENGER-RNA AND TRKB PROTEIN-LIKE IMMUNOREACTIVITY IN THE INJURED RAT AND CAT SPINAL-CORD
INCREASED LEVELS OF TRKB MESSENGER-RNA AND TRKB PROTEIN-LIKE IMMUNOREACTIVITY IN THE INJURED RAT AND CAT SPINAL-CORD
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DOI：
10.1073/pnas.89.23.11282
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发表时间：
1992-12-01
影响因子：
11.1
通讯作者：
RISLING, M
中科院分区：
文献类型：
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作者：
FRISEN, J;VERGE, VMK;RISLING, M
神经营养因子和神经营养因子受体的表达进行了研究，在腹侧或背索脊髓病变后10天至6周，在成年大鼠和猫，病变，以前已被证明允许轴突再生与原位杂交和免疫组织化学技术。在两种类型的病变后，在白色物质中形成的瘢痕组织中观察到trkB mRNA水平的强烈升高。神经生长因子、脑源性神经营养因子、神经营养因子3、神经营养因子4、trk和trkC mRNA对损伤的反应仅略有增加。trkB蛋白样免疫反应性在显示trkB mRNA水平升高的区域中增加。EM定位于神经元，星形胶质细胞和软脑膜细胞的免疫反应。神经丝免疫标记和轴突追踪表明，瘢痕组织中的神经纤维集中在显示出强烈的trkB蛋白样免疫反应性的区域。这些发现暗示了神经营养因子受体在受损脊髓轴突发芽和神经胶质反应中的作用。
Expression of neurotrophins and neurotrophin receptors was examined with in situ hybridization and immunohistochemical techniques 10 days to 6 weeks after ventral or dorsal funiculus spinal cord lesions in adult rats and cats, lesions that have previously been shown to allow axon regrowth. Strongly elevated levels of trkB mRNA were seen in the scar tissue formed in the white matter after both types of lesions. Only small increases were detected for nerve growth factor, brain-derived neurotrophic factor, neurotrophin 3, neurotrophin 4, trk, and trkC mRNA in response to the injuries. trkB protein-like immunoreactivity was increased in the regions that showed elevated levels of trkB mRNA. EM localized this immunoreactivity to neurons, astrocytes, and leptomeningeal cells. Neurofilament immunolabeling and axonal tracing demonstrated that nerve fibers in the scar tissue were concentrated to areas that showed strong trkB protein-like immunoreactivity. The findings implicate a role for neurotrophin receptors in axonal sprouting and glial reactions in the injured spinal cord.