Light-Responsive Polyion Complex Micelles with Switchable Surface Charge for Efficient Protein Delivery
Light-Responsive Polyion Complex Micelles with Switchable Surface Charge for Efficient Protein Delivery
复制标题
具有可切换表面电荷的光响应聚离子复合胶束，可实现高效蛋白质递送
DOI：
10.1021/mz500290s
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发表时间：
2014-07-01
影响因子：
7.015
通讯作者：
Ji, Jian
中科院分区：
文献类型：
--
作者：
Jin, Qiao;Cai, Tongjiang;Ji, Jian
本文采用阳离子嵌段共聚物聚（N，N-二甲基-N-（2-（甲基丙烯酰氧基）乙基）-N-（（2-硝基苄基）氧基）-2-氧代乙基溴化铵）-嵌段-聚（羧基甜菜碱甲基丙烯酸酯）（PDMNBMA-b-PCBMA）与带负电荷的牛血清白蛋白（BSA）自组装，制备了光响应性蛋白质包封聚离子复合物（PIC）胶束。用动态光散射（DLS）和透射电子显微镜（TEM）对PIC胶束进行了表征。从zeta电位的测量，PIC胶束的zeta电位从接近10增加到接近20 mV时，观察到溶液pH值从7.4降低到6.5，这可以提高细胞内的蛋白质递送效率。此外，带正电荷的PDMNBMA块可以在紫外光照射下转化为两性离子羧基甜菜碱单元，这可能导致PIC胶束的解体。因此，在UV照射的存在下，BSA的释放可以显著加速。同时，圆二色性（CD）光谱证实，在紫外光照射过程中的BSA的二级结构不受影响。
In this letter, light-responsive protein-encapsulated polyion complex (PIC) micelles were prepared by self-assembly of cationic block copolymer poly(N,N-dimethyl-N-(2-(methacryloyloxy)ethyl)-N-((2-nitrobenzyl)oxy)-2-oxoe-thanaminium bromide)-block-poly(carboxybetaine methacrylate) (PDMNBMA-b-PCBMA) and negatively charged bovine serum albumin (BSA). The PIC micelles were well characterized by dynamic light scattering (DLS) and transmission electron microscopy (TEM). From the zeta potential measurement, the increase of the zeta potential of PIC micelles from similar to 10 to similar to 20 mV was observed when the solution pH decreased from 7.4 to 6.5, which could enhance the intracellular protein delivery efficiency. Moreover, the positively charged PDMNBMA blocks can be transformed to zwitterionic carboxybetaine units under UV irradiation, which could result in the disassembly of the PIC micelles. The release of BSA can therefore be drastically accelerated in the presence of UV irradiation. Meanwhile, the circular dichroism (CD) spectroscopy confirmed that the secondary structure of BSA was unaffected during the UV irradiation process.