Aging-dependent expression of synapse-related proteins in the mouse brain.
Aging-dependent expression of synapse-related proteins in the mouse brain.
复制标题
小鼠大脑中突触相关蛋白的衰老依赖性表达。
作者：
Shiotani;H.;Maruo;T.;Sakakibara;S.;Miyata;M.;Mandai;K.;Mochizuki;H.;Takai;Y.
突触是一种细胞粘附结构，允许神经元将化学或电信号传递给另一个神经元。它们连接神经元并形成神经网络，这些神经网络对大脑功能（如学习和记忆）至关重要。在化学突触中，突触前和突触后由细胞粘附分子连接。突触前包含突触囊泡及其释放机制，而突触后包含突触后密度和神经递质受体。许多构成突触的蛋白质已被鉴定，但它们的寿命表达谱仍然难以捉摸。在这里，我们使用不同年龄小鼠脑的细胞核上清液部分，通过Western印迹法研究了代表性突触相关蛋白的表达水平。这些蛋白质被分为7组，根据其表达谱在胚胎阶段，那些从出生后第6天（P6）至P30，和那些后P90。大多数蛋白质的表达水平从胚胎期开始逐渐增加，然后在P14或P30下降。P90后，表达水平没有明显变化，或在某些蛋白质中，增加。这些结果表明，突触相关蛋白的表达水平以衰老依赖的方式有序调节。
A synapse is a cell adhesion structure that permits a neuron to pass a chemical or electrical signal to another neuron. They connect neurons and form neural networks that are essential for brain functions, such as learning and memory. At a chemical synapse, the presynapse and the postsynapse are connected by cell adhesion molecules. The presynapse contains synaptic vesicles and their release machinery, whereas the postsynapse contains postsynaptic densities and receptors for the neurotransmitters. Many proteins constituting a synapse have been identified, but their life‐span expression profiles remain elusive. Here, we investigated the expression levels of representative synapse‐related proteins by Western blot using the extranuclear supernatant fraction of the brains of mice at various ages. These proteins were classified into seven groups depending on their expression profiles during the embryonic stage, those from postnatal day 6 (P6) to P30, and those after P90. The expression levels of the majority of the proteins were gradually increased from the embryonic stage and then decreased at P14 or P30. After P90, the expression levels were not markedly changed or, in some proteins, increased. These results indicate that the expression levels of the synapse‐related proteins are regulated orderly in an aging‐dependent manner.