Polysaccharides from Dioscorea batatas Induce Tumor Necrosis Factor-α Secretion via Toll-like Receptor 4-Mediated Protein Kinase Signaling Pathways
Polysaccharides from Dioscorea batatas Induce Tumor Necrosis Factor-α Secretion via Toll-like Receptor 4-Mediated Protein Kinase Signaling Pathways
复制标题
DOI：
10.1021/jf8018722
复制
发表时间：
2008-11-12
影响因子：
6.1
通讯作者：
Wu, Shih-Hsiung
中科院分区：
文献类型：
--
作者：
Liu, Ju-Yun;Yang, Feng-Ling;Wu, Shih-Hsiung
从山药中提取的生物活性多糖（ZPF 1）经化学分析表明，重复β-1，4-甘露聚糖主要以C-2-OH和C-3-OH的乙酰化为特征，其含量约为28%。ZPF 1主要参与刺激肿瘤坏死因子-α（TNF α）中的鼠野生型巨噬细胞。Toll样受体4被证明是ZPF 1介导的TNF α分泌的细胞受体之一。活性氧传递和P13-激酶被发现是通过ZPF 1刺激调节TNF α分泌所必需的。此外，我们发现，细胞外信号调节激酶1/2，Jun N-末端激酶1/2，和p38丝裂原活化蛋白激酶在ZPF 1刺激的巨噬细胞的TNF α分泌的调节中发挥重要作用。
The bioactive polysaccharides (named ZPF1) from yam (Dioscorea batatas) were chemically determined, suggesting repeating beta-1,4-mannan as mainly having a feature of acetylation on C-2-OH and C-3-OH, around 28%. The ZPF1 participated in the stimulation of murine wild-type macrophages predominantly in tumor necrosis factor-alpha (TNF alpha). Toll-like receptor 4 is proved to be one of the cellular receptors in ZPF1-mediated TNF alpha secretion. Reactive oxygen species transmission and P13-kinase are found necessary for regulating TNF alpha secretion by ZPF1 stimulation. Moreover, we found that extracellular signal-regulated kinase 1/2, Jun N-terminal kinase 1/2, and p38 mitogen-activated protein kinase play important roles in the regulation of TNF alpha secretion in ZPF1-stimulated macrophages.