A Phylogenetic and Functional Perspective on Volatile Organic Compound Production by Actinobacteria
A Phylogenetic and Functional Perspective on Volatile Organic Compound Production by Actinobacteria
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DOI：
10.1128/msystems.00295-18
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发表时间：
2019-03-01
期刊：
影响因子：
6.4
通讯作者：
Fierer, Noah
中科院分区：
文献类型：
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作者：
Choudoir, Mallory;Rossabi, Sam;Fierer, Noah
土壤微生物产生多种多样的代谢产物，包括挥发性有机化合物（VOCs），它们可以塑造微生物群落的结构和功能。VOC介导多种微生物-微生物相互作用，包括拮抗作用。尽管它们的重要性，大多数微生物挥发物的多样性和功能相关性仍然是未知的。我们组装了一个分类学上不同的收集48放线菌分离土壤和空气中的灰尘和调查的挥发性有机化合物，这些菌株在两种不同的介质类型在体外使用气相色谱-质谱（GC-MS）。我们检测到了126种不同的VOC，并在结构上鉴定了其中约20%的化合物，主要是C-1至C-5杂VOC，包括（含氧）醇，酮，酯，以及含氮和含硫化合物。每种菌株产生独特的VOC谱。虽然最常见的VOC可能是初级代谢的副产物，但大多数VOC都是菌株特异性的。我们观察到一个强大的分类和系统发育的VOC配置文件的信号，这表明他们的作用，在更细的尺度模式的生态多样性。最后，我们研究了这些挥发性有机化合物的功能潜力，通过评估其对致病性和非致病性假单胞菌菌株的生长速度的影响。我们确定了与生长抑制和刺激相关的挥发性有机化合物集，这些信息可能有助于开发基于微生物挥发性有机化合物的病原体控制strategies.IMPORTANCE土壤微生物产生各种各样的天然产物，包括挥发性有机化合物（VOCs）。挥发性化合物是土壤生境中的重要分子，在那里它们介导细菌、真菌、昆虫、植物和动物之间的相互作用。我们测量的挥发性有机化合物产生的广泛多样性的土壤和灰尘居住放线菌在体外。我们总共检测到126种独特的挥发性化合物，每种菌株都产生了独特的VOC组合。虽然一些化合物由许多菌株产生，但大多数是菌株特异性的。重要的是，挥发性有机化合物配置文件之间的密切相关的菌株更相似，表明进化和生态过程产生可预测的模式的挥发性有机化合物的生产。最后，我们观察到放线菌挥发性有机化合物对代表植物有益共生体和植物致病菌株的细菌的生长具有刺激和抑制作用，这些信息可能导致植物病害预防新策略的发展。
Soil microbes produce an immense diversity of metabolites, including volatile organic compounds (VOCs), which can shape the structure and function of microbial communities. VOCs mediate a multitude of microbe-microbe interactions, including antagonism. Despite their importance, the diversity and functional relevance of most microbial volatiles remain uncharacterized. We assembled a taxonomically diverse collection of 48 Actinobacteria isolated from soil and airborne dust and surveyed the VOCs produced by these strains on two different medium types in vitro using gas chromatography-mass spectrometry (GC-MS). We detected 126 distinct VOCs and structurally identified approximately 20% of these compounds, which were predominately C-1 to C-5 hetero-VOCs, including (oxygenated) alcohols, ketones, esters, and nitrogen-and sulfur-containing compounds. Each strain produced a unique VOC profile. While the most common VOCs were likely by-products of primary metabolism, most of the VOCs were strain specific. We observed a strong taxonomic and phylogenetic signal for VOC profiles, suggesting their role in finer-scale patterns of ecological diversity. Finally, we investigated the functional potential of these VOCs by assessing their effects on growth rates of both pathogenic and non-pathogenic pseudomonad strains. We identified sets of VOCs that correlated with growth inhibition and stimulation, information that may facilitate the development of microbial VOC-based pathogen control strategies.IMPORTANCE Soil microbes produce a diverse array of natural products, including volatile organic compounds (VOCs). Volatile compounds are important molecules in soil habitats, where they mediate interactions between bacteria, fungi, insects, plants, and animals. We measured the VOCs produced by a broad diversity of soil- and dust-dwelling Actinobacteria in vitro. We detected a total of 126 unique volatile compounds, and each strain produced a unique combination of VOCs. While some of the compounds were produced by many strains, most were strain specific. Importantly, VOC profiles were more similar between closely related strains, indicating that evolutionary and ecological processes generate predictable patterns of VOC production. Finally, we observed that actinobacterial VOCs had both stimulatory and inhibitory effects on the growth of bacteria that represent a plant-beneficial symbiont and a plant-pathogenic strain, information that may lead to the development of novel strategies for plant disease prevention.