Gait abnormalities differentially indicate pain or structural joint damage in monoarticular antigen-induced arthritis
Gait abnormalities differentially indicate pain or structural joint damage in monoarticular antigen-induced arthritis
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DOI：
10.1016/j.pain.2009.06.006
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发表时间：
2009-09-01
期刊：
影响因子：
7.4
通讯作者：
Schaible, Hans-Georg
中科院分区：
文献类型：
--
作者：
Boettger, Michael K.;Weber, Konstanze;Schaible, Hans-Georg
步态异常被认为是动物模型中关节疼痛的客观测量指标。在这里，我们的目的是评估步态分析参数是否与实验性单关节炎疼痛相关行为的测量相关。为此，我们在68只雌性Lewis大鼠的左膝关节中诱导了抗原诱导的关节炎，其中30只用肿瘤坏死因子- α (TNF)中和化合物治疗。在关节炎过程中，获得机械痛敏和热痛敏的爪印分析参数和措施。在第3天和第21天采集的膝关节进行组织学评分，以确定炎症和软骨和骨破坏的迹象，并将数据与33只免疫对照大鼠的数据进行比较，并在第3天和第21天进行相关。关节炎大鼠表现出明显的不对称步态异常。在抗原诱导关节炎的急性期，而非慢性期，步态参数“左右距离”与原发性和继发性痛觉过敏之间存在显著相关性。然而，在急性和慢性阶段，步态参数“两爪之间的角度”表明爪子向外旋转，主要与关节破坏相关，通过组织学评估。依那西普治疗表现出明显的抗伤害性和促进运动作用，但上述相关性仍然存在。总之，步态分析的一些参数可以很好地衡量关节炎疼痛，主要是在急性炎症中，而其他参数则越来越多地受到关节机械变形的影响，如软骨和骨破坏。因此，步态异常可能不明确适用于所有阶段的实验性关节炎的客观疼痛评估。(C) 2009年国际疼痛研究协会。Elsevier B.V.版权所有。
Gait abnormalities have been suggested to provide an objective measure for joint pain in animal models. Here, we aimed to assess whether parameters of gait analysis correlate with measures of pain-related behavior in experimental monoarthritis. For this purpose, antigen-induced arthritis was induced in the left knee joints of 68 female Lewis rats, of which 30 were treated with tumor necrosis factor-alpha(TNF)-neutralizing compounds. During the course of arthritis, paw print analysis parameters and measures for mechanical and thermal hyperalgesia were obtained. Knee joints harvested on either day 3 or day 21 were scored histologically for signs of inflammation and cartilage and bone destructions, Data were compared to those obtained from 33 immunized control rats and correlated for days 3 and 21. Arthritic rats showed distinct asymmetric gait abnormalities. In the acute stage of antigen-induced arthritis, but not in the chronic phase, there was a significant correlation between the gait parameter 'left-right distance' and measures of primary and secondary hyperalgesia. Both in the acute and chronic phases, however, the gait parameter 'angle between paws' indicating outward rotation of paws mainly correlated with joint destruction as assessed using histology. Etanercept treatment exhibited pronounced anti-nociceptive and pro-locomotional effects, but the described correlations remained. In conclusion, some parameters of gait analysis may represent a good measure for arthritis pain, mainly in acute inflammation, while others are increasingly influenced by mechanical joint deformation as indicated by cartilage and bone destructions. Thus, gait abnormalities may not unequivocally be suitable for objective pain assessment in all stages of experimental arthritis. (C) 2009 International Association for the Study of Pain. Published by Elsevier B.V. All rights reserved.