Involvement and necessity of the Cpx regulon in the event of aberrant beta-barrel outer membrane protein assembly.
Involvement and necessity of the Cpx regulon in the event of aberrant beta-barrel outer membrane protein assembly.
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DOI：
10.1111/j.1365-2958.2009.07042.x
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发表时间：
2010-02
影响因子：
3.6
通讯作者：
Misra R
中科院分区：
文献类型：
--
作者：
Gerken H;Leiser OP;Bennion D;Misra R
The Cpx and σE regulons help maintain outer membrane integrity; the Cpx pathway monitors the biogenesis of cell surface structures, such as pili, while the σE pathway monitors the biogenesis of β-barrel outer membrane proteins (OMPs). In this study we revealed the importance of the Cpx regulon in the event of β-barrel OMP mis-assembly, by utilizing mutants expressing either a defective β-barrel OMP assembly machinery (Bam) or assembly defective β-barrel OMPs. Analysis of specific mRNAs showed that ΔcpxR bam double mutants failed to induce degP expression beyond the wild type level, despite activation of the σE pathway. The synthetic conditional lethal phenotype of ΔcpxR in mutant Bam or β-barrel OMP backgrounds was reversed by wild type DegP expressed from a heterologous plasmid promoter. Consistent with the involvement of the Cpx regulon in the event of aberrant β-barrel OMP assembly, the expression of cpxP, the archetypal member of the cpx regulon, was upregulated in defective Bam backgrounds or in cells expressing a single assembly-defective β-barrel OMP species. Together, these results showed that both the Cpx and σE regulons are required to reduce envelope stress caused by aberrant β-barrel OMP assembly, with the Cpx regulon principally contributing by controlling degP expression.
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影响因子：
3.2
作者：
Batchelor, E;Walthers, D;Goulian, M
通讯作者：
Goulian, M
影响因子：
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GROSS, CA
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通讯作者：
Raina, S
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作者：
CHANG, ACY;COHEN, SN
通讯作者：
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