Molecular characterization of the Kruppel-homolog 1 and its role in ovarian development in Sogatella furcifera (Hemiptera: Delphacidae)
Molecular characterization of the Kruppel-homolog 1 and its role in ovarian development in Sogatella furcifera (Hemiptera: Delphacidae)
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Krüppel-同源物 1 的分子特征及其在白背飞虱（半翅目：飞虱科）卵巢发育中的作用
DOI：
10.1007/s11033-019-05206-7
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发表时间：
2019-11-29
影响因子：
2.8
通讯作者：
Li, Youzhi
中科院分区：
文献类型：
--
作者：
Hu, Kui;Tian, Ping;Li, Youzhi
保幼激素（JH）在昆虫生殖过程中起着重要作用。Kruppel同源物1（Kr-h1）是一种JH诱导的锌指转录因子，也被发现在昆虫繁殖中发挥作用，然而，其功能因物种而异。本研究克隆了白背飞虱SfKr-h1基因，并研究了其在卵巢发育中的作用。SfKr-h1开放阅读框为1 800 bp，编码599个氨基酸。SfKr-h1的推定氨基酸序列含有8个推定的C2 H2型锌指结构域，与其他半翅目昆虫Kr-h1 s高度同源。SfKr-h1的表达在成虫羽化后96 h达到高峰，在卵巢中的表达最高。RNA干扰（RNAi）敲低SfKr-h1显著降低了SfVg的转录，并阻止了卵巢发育。这些结果表明，SfKr-h1是关键的正常卵巢发育在S。furcifera。
Juvenile hormone (JH) plays a pivotal role in insect reproduction. The Kruppel-homolog 1 (Kr-h1) is a JH-inducible zinc finger transcription factor that has also been found to play a role in insect reproduction, however, its function varies across species. In this study, we cloned SfKr-h1 from Sogatella furcifera and investigated its role in ovarian development. The open reading frame of SfKr-h1 is 1 800 bp encoding 599 amino acids. The putative amino acid sequence of SfKr-h1 contains eight putative C2H2-type zinc finger domains and is highly homologous with the Kr-h1s of other hemipteran species. Expression of SfKr-h1 peaked 96 h after adult emergence and was highest in the ovary. RNA interference (RNAi) knockdown of SfKr-h1 substantially reduced the transcription of SfVg, and arrested ovarian development. These results suggest that SfKr-h1 is critical for normal ovarian development in S. furcifera.