Persistent myocardial ischemia increases GLUT1 glucose transporter expression in both ischemic and non-ischemic heart regions.
Persistent myocardial ischemia increases GLUT1 glucose transporter expression in both ischemic and non-ischemic heart regions.
复制标题
持续性心肌缺血会增加缺血和非缺血心脏区域的 GLUT1 葡萄糖转运蛋白表达。
DOI：
10.1006/jmcc.1997.0405
复制
发表时间：
1997
影响因子：
5
通讯作者：
Schwaiger,M
中科院分区：
文献类型：
--
作者：
Brosius3rd,FC;Liu,Y;Nguyen,N;Sun,D;Bartlett,J;Schwaiger,M
持续缺血心肌表现出葡萄糖摄取增加，这可能有助于心肌功能和活力的保存。对于导致摄取增加的具体分子事件知之甚少。因此，我们研究心肌缺血是否会诱导心肌促性葡萄糖转运蛋白GLUT4和GLUT1的基因表达。采用半定量Northern blotting和免疫印迹法检测犬局部缺血6 h后心肌葡萄糖转运蛋白mrna和多肽的表达。与手术对照组和正常心脏相比，实验心脏缺血区和非缺血区GLUT1的表达均显著增加，而GLUT4的表达不显著增加。与正常或手术对照心脏相比，缺血心脏中GLUT1 mRNA表达增加3.4倍，GLUT1多肽表达增加1.7倍。GLUT1在正常心脏和缺血心脏中的表达无显著的区域差异。然而，在缺血6小时心脏的非缺血区域，GLUT1 mRNA的表达有最高的趋势。这些发现提示心肌缺血诱导一个或多个因子刺激GLUT1在非缺血和缺血心肌区域的表达。升高的GLUT1表达可能在缺血时增加葡萄糖摄取中起作用。
Persistently ischemic myocardium exhibits increased glucose uptake which may contribute to the preservation of myocardial function and viability. Little is known about the specific molecular events which are responsible for this increase in uptake. Therefore, we investigated whether myocardial ischemia induces the gene expression of the major cardiac facilitative glucose transporters, GLUT4 and GLUT1. We determined the expression of myocardial glucose transporter mRNAs and polypeptides after 6 h of regional ischemia in a dog model by semi-quantitative Northern blotting and immunoblotting. GLUT1 but not GLUT4 expression was significantly increased in both ischemic and non-ischemic regions from the experimental hearts when compared to surgical control and normal hearts. GLUT1 mRNA expression was increased 3.4-fold and GLUT1 polypeptide expression was increased 1.7-fold in ischemic hearts when compared to normal or surgical-control hearts. There were no significant regional differences in GLUT1 expression in either normal or ischemic hearts. However, there was a tendency for GLUT1 mRNA expression to be highest in the non-ischemic regions from the 6-h ischemia hearts. These findings suggest that myocardial ischemia induces a factor or factors which stimulate GLUT1 expression in non-ischemic as well as ischemic myocardial regions. Increased GLUT1 expression may play a role in augmenting glucose uptake during ischemia.