An up Conversion Optical System Based on Mesoporous Silica Encapsulated Up-Converting Nanoparticles Labeled Lateral Flow Immunoassay for Procalcitonin Quantification in Plasma
An up Conversion Optical System Based on Mesoporous Silica Encapsulated Up-Converting Nanoparticles Labeled Lateral Flow Immunoassay for Procalcitonin Quantification in Plasma
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DOI：
10.1109/jstqe.2021.3060496
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发表时间：
2021-09-01
影响因子：
4.9
通讯作者：
Guo, Jinhong
中科院分区：
文献类型：
--
作者：
Guo, Jiuchuan;Zhang, Jiechen;Guo, Jinhong
随着纳米技术的发展，基于纳米材料的生物传感器得到了广泛的发展。其中，光学生物传感器因其灵敏度高、检测速度快而脱颖而出。在本文中，我们展示了一种基于免疫色谱的上转换发光（UCL）系统，通过使用核壳介孔二氧化硅封装的上转换纳米粒子（UCNPs@mSiO（2））作为探针定量检测血浆中的降钙素原（PCT）。与其他结构相比，UCNPs@mSiO（2）具有良好的生物相容性和较强的发光强度，是一种非常理想的免疫检测材料。我们进一步设计了一个便携式UCL阅读器，用于光信号的采集和处理。结果表明，基于UCNPs@mSiO（2）的侧向流动系统可定量PCT浓度，范围为1-200 ng/mL，检测限为0.5 ng/mL。我们相信这种UCL系统在感染的监测和早期诊断方面具有很大的潜力。
With the advancing of nanotechnology, nanomaterials based biosensors have been widely developed. Among them, optical biosensors stand out due to the high sensitivity and fast detection. In this paper, we demonstrate an up conversion luminescence (UCL) system based on immunochromatography by using core-shell mesoporous silica encapsulated up-converting nanoparticles (UCNPs@mSiO(2)) as probes for quantitative detection of Procalcitonin (PCT) in plasma. Compared with other structures, UCNPs@mSiO(2) possess both great biocompatibility and strong luminous intensity, which is extremely ideal in immune detection. We further design a portable UCL reader for optical signal collecting and processing. As a result, the UCNPs@mSiO(2) based lateral flow system can quantify PCT concentration with a range of 1-200 ng/mL, and the limit of detection is 0.5 ng/mL. We believe this UCL system possesses a promising potential in the monitoring and early diagnosis of infections.