A COMMON FUNCTION FOR MESSENGER-RNA-5' AND MESSENGER-RNA-3' ENDS IN TRANSLATION INITIATION IN YEAST
A COMMON FUNCTION FOR MESSENGER-RNA-5' AND MESSENGER-RNA-3' ENDS IN TRANSLATION INITIATION IN YEAST
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DOI：
10.1101/gad.9.23.2997
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发表时间：
1995-12-15
影响因子：
10.5
通讯作者：
SACHS, AB
中科院分区：
文献类型：
--
作者：
TARUN, SZ;SACHS, AB
Poly(A)Tail及其相关的Poly(A)结合蛋白(Pab1p)在真核生物中普遍存在。Poly(A)尾巴的功能是稳定mRNA并刺激其翻译。依赖PolyA和帽的酵母体外翻译系统的发展使我们能够了解PolyA是如何刺激翻译的。我们发现Pab1p而不是帽结合蛋白eIF-4E是Poly(A)尾依赖翻译所必需的，并且Pab1p-Poly(A)尾巴复合体具有将40S核糖体亚基招募到mRNA的功能。这些数据为翻译起始的途径引入了新的一步，并融合了mRNA两端的翻译功能。
The mRNA poly(A) tail and its associated poly(A) binding protein (Pab1p) are ubiquitous in eukaryotes. The function of the poly(A) tail is to stabilize mRNA and to stimulate its translation The development of a poly(A)- and cap-dependent yeast in vitro translation system has allowed us to understand how poly(A) stimulates translation. We find that Pab1p but not the cap binding protein eIF-4E is required for poly(A) tail dependent translation, and that the Pab1p-poly(A) tail complex functions to recruit the 40S ribosomal subunit to the mRNA. These data introduce a new step into the pathway of translation initiation and merge the translational functions of the two ends of mRNA.