EXPRESSION OF THE T-CELL SURFACE-MOLECULE CD2 AND AN EPITOPE-LOSS CD2 MUTANT TO DEFINE THE ROLE OF LYMPHOCYTE FUNCTION-ASSOCIATED ANTIGEN 3 (LFA-3) IN T-CELL ACTIVATION
EXPRESSION OF THE T-CELL SURFACE-MOLECULE CD2 AND AN EPITOPE-LOSS CD2 MUTANT TO DEFINE THE ROLE OF LYMPHOCYTE FUNCTION-ASSOCIATED ANTIGEN 3 (LFA-3) IN T-CELL ACTIVATION
复制标题
DOI：
10.1073/pnas.85.4.1194
复制
发表时间：
1988-02-01
影响因子：
11.1
通讯作者：
BURAKOFF, SJ
中科院分区：
文献类型：
--
作者：
BIERER, BE;PETERSON, A;BURAKOFF, SJ
为了确定 CD2 淋巴细胞功能相关抗原 3 (LFA-3) 相互作用在 T 细胞激活中的作用，我们在鼠类抗原特异性 T 细胞杂交瘤中表达了编码人 CD2 分子的 cDNA。 CD2分子的表达大大增强了T细胞对抗原的反应性；这种增强作用可被抗 CD2 和抗 LFA-3 单克隆抗体 (mAb) 抑制。 CD2+杂交瘤响应抗CD2 mAb 9.6和9-1的组合产生白细胞介素2，并且在mAb 9-1存在的情况下，响应绵羊红细胞或LFA-3抗原。此外，表达已失去mAb9.6结合的突变型CD2分子的杂交瘤不表现出对抗原的增强的应答或对LFA-3加mAb 0-1的应答能力，但这些杂交瘤保留了对抗CD2 mAb组合的应答能力。讨论了 CD2-LFA-3 相互作用在 T 细胞激活中的作用以及 CD2 的其他生理配体的潜力。
To define the role of the CD2-lymphocyte function-associated antigen 3 (LFA-3) interaction in T-cell activation, we have expressed a cDNA encoding the human CD2 molecule in amurine antigen-specific T-cell hybridoma. Expression of the CD2 molecule greatley enhances t-cell responsiveness to antigen; this enhancement is inhibited by anti-CD2 and anti-LFA-3 monoclonal antibodies (mAbs). CD2+ hybridomas produce interleukin 2 in response to combinations of anti-CD2 mAbs 9.6 and 9-1 and, in the presence of mAb 9-1, to sheep erythrocytes or to the LFA-3 antigen. Furthermore, hybridomas expressing a mutant CD2 molecule that has lost mAb9.6 binding do not exhibit the enhanced response to antigen or the ability to respond to LFA-3 plus mAb 0-1, but these hybridomas retain the ability to respond to combinations of anti-CD2 mAbs. The role of the CD2-LFA-3 interaction in T-cell activation and the potential for other physiologic ligands for CD2 are discussed.