Comparison of the natural fluorescence distribution among size fractions of terrestrial fulvic and humic acids and aquatic natural organic matter
Comparison of the natural fluorescence distribution among size fractions of terrestrial fulvic and humic acids and aquatic natural organic matter
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DOI：
10.1016/j.orggeochem.2004.06.010
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发表时间：
2004-01-01
影响因子：
3
通讯作者：
Takács, M
中科院分区：
文献类型：
--
作者：
Alberts, JJ;Takács, M
用高效排斥色谱(HPSEC)分离了土壤黄腐酸(SOILFA)、土壤腐植酸(SOILHA)和水生天然有机物(SUWNOM)样品。收集各组分，测定各组份的同步扫描光谱和总发光荧光(TL)光谱。同时扫描的光谱很难解释，因为强调光谱中的峰肩而不是峰最大值。总发光光谱表明，尽管紫外吸收碳在级分中的粒度分布不同，但所有三个样品的荧光产生部分都出现在相同的、相对较小的级分中。对于SOILFA和SUWNOM，95%的紫外吸收碳与95%的荧光符合。然而，在SOILHA中，只有大约50%的紫外吸收碳与90%的荧光符合。由于紫外光吸收和荧光碳在相同尺寸组分中的高符合率(>；95%)，原位荧光可能为跟踪天然有机物在水生系统中的运动提供了希望。(C)2004爱思唯尔有限公司。保留所有权利。
A soil fulvic acid (SOILFA), a soil humic acid (SOILHA) and an aquatic natural organic matter (SUWNOM) sample were separated into a spectrum of size fractions with high performance size exclusion chromatography (HPSEC). Fractions were collected and the synchronously scanned and total luminescence fluorescence (TL) spectra were determined for each fraction. Synchronously scanned spectra were difficult to interpret due to the emphasis placed on peak shoulders in the spectra rather than peak maxima. Total luminescence spectra indicate that despite differences in the size distributions of UV absorbing carbon in the fractions, the fluorescence producing moieties of all three samples occur in the same, relatively small size fractions. For the SOILFA and the SUWNOM, 95% of the UV absorbing carbon was coincident with 95% of the fluorescence. However, in the SOILHA, only about 50% of the UV absorbing carbon was coincident with 90% of the fluorescence. Due to the high (>95%) coincidence of UV absorbing and fluorescent carbon in the same size fractions, in situ fluorescence may offer promise for following the movement of natural organic matter in aquatic systems. (C) 2004 Elsevier Ltd. All rights reserved.