Developmental changes in growth factors released by the embryonic inner ear.
Developmental changes in growth factors released by the embryonic inner ear.
复制标题
胚胎内耳释放的生长因子的发育变化。
DOI：
10.1006/exnr.1997.6774
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
Cohan,CS
中科院分区：
文献类型：
--
作者：
Bianchi,LM;Dolnick,R;Medd,A;Cohan,CS
最近的研究已经证明了神经营养因子在调节发育中的听觉和前庭神经元的存活中的作用。然而，内耳组织产生和释放神经营养因子的发育时间过程尚未确定。在本研究中，神经营养素样活性进行了评估，从培养液条件的早期或中期阶段内耳。条件培养基的蛋白质性质的检查揭示了内耳生长因子释放的发育变化。神经营养因子样分子在早期耳囊肿条件培养基中未检测到。相反，在发育中期内耳的条件培养基中检测到神经营养素样生物活性。Western blot分析显示，NT-3在大鼠内耳发育中期由内耳释放。ELISA测定结果表明，NT-3和BDNF都是由内耳发育中期产生的，并且NT-3蛋白水平高于BDNF水平。这些结果表明，有发展变化的释放生长因子的内耳。
Recent studies have demonstrated a role for neurotrophins in regulating the survival of developing auditory and vestibular neurons. However, the developmental time-course for neurotrophin production and release by inner ear tissues has not been defined. In the present study, neurotrophin-like activity was evaluated from culture medium conditioned by early- or midembryonic stage inner ears. Examination of the proteinaceous properties of conditioned medium revealed a developmental change in growth factor release by the inner ear. Neurotrophin-like molecules were not detected in medium conditioned by early stage otocysts. In contrast, neurotrophin-like bioactivity was detected in medium conditioned by middevelopmental stage inner ears. Western blot analysis revealed that NT-3 was released by the rat inner ear at midstages of inner ear development. ELISA measurements revealed that both NT-3 and BDNF are produced by the middevelopmental stage inner ear, and that NT-3 protein levels are higher than BDNF levels. These results suggest that there are developmental changes in the release of growth factors by the inner ear.