Continuous monitoring of tissue growth inside a perfusion bioreactor by optical coherence tomography
Continuous monitoring of tissue growth inside a perfusion bioreactor by optical coherence tomography
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通过光学相干断层扫描连续监测灌注生物反应器内的组织生长
DOI：
10.1117/12.701052
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发表时间：
2007
期刊：
影响因子：
--
通讯作者：
Y. Yang
中科院分区：
文献类型：
--
作者：
P. Bagnaninchi;A. E. El Haj;Y. Yang
组织工程的目的是创造体外功能组织，最终可以用作自体移植物。考虑到必须测试大量参数以优化组织生长并实现对组织形成的更好理解，需要相关的生物体外模型，其可以沿着组织工程的所有沿着不同阶段进行监测和表征：细胞接种、细胞生长、细胞外基质（ECM）沉积、基质翻转和组织组织化。我们开发了多孔壳聚糖支架（1.5 mm），其封闭300 m微通道以促进流体剪切应力刺激，更具体地说，以支持肌腱组织工程的特定情况下的束形成。将这些支架装载在灌注生物反应器中，并通过光学相干断层扫描（OCT）在几天内进行监测。基于光纤的时域OCT采用1300nm的超辐射发光二极管，其带宽为52nm，在自由空间中的xyz分辨率为16*16*14。这种设置使我们能够评估接种在微通道中的细胞的体积分数，从而优化接种程序。在不同的时间点成功地监测了细胞生长和ECM沉积，因为通道被新形成的材料填充。在细胞生长和ECM产生过程中观察到不同的散射行为。在灌注下连续监测支架的可能性将允许容易地辨别影响预组织形成速率生长的参数。
Tissue engineering aims to create in vitro functional tissues that could ultimately be used as autologous implants. Considering the large number of parameters that have to be tested to optimize the tissue growth and to achieve a better understanding of tissue formation, relevant biological in vitro models are needed which can be monitored and characterized all along the different stages of tissue engineering: cell seeding, cell growth, extra-cellular matrix (ECM) deposition, matrix turn-over and tissue organization. We developed porous chitosan scaffolds (&fgr;1.5mm) that enclose a 300&mgr;m microchannel to encourage fluid shear-stress stimulation and more specifically to support bundle formation for the specific case of tendon tissue engineering. These scaffolds were loaded in perfusion bioreactors and monitored during several days by optical coherence tomography (OCT). The fiber based time domain OCT employed a 1300nm superluminescent diode with a bandwith of 52 nm and a xyz resolution of 16*16*14 in free space. This set up allowed us to assess the volume fraction of cell seeded in the microchannels, and thus to optimize the seeding procedure. The cell growth and ECM deposition were successfully monitored at different time point as the channels were filled by newly formed material. Different scattering behaviors have been observed during cell growth and ECM production. The possibility to monitor continuously the scaffolds under perfusion will allow an easy discrimination of the parameters affecting the pre-tissue formation rate growth.
发育生物学中的光学相干断层扫描成像。
DOI：
10.1385/1-59259-685-1:217
发表时间：
2000
期刊：
Methods in molecular biology (Clifton, N.J.)
影响因子：
--
作者：
Boppart,SA;Brezinski,ME;Fujimoto,JG
通讯作者：
Fujimoto,JG