NUCLEAR SIZE AS A CELL-KINETIC MARKER FOR OSTEOBLAST DIFFERENTIATION
NUCLEAR SIZE AS A CELL-KINETIC MARKER FOR OSTEOBLAST DIFFERENTIATION
复制标题
DOI：
10.1002/aja.1001650403
复制
发表时间：
1982-01-01
影响因子：
--
通讯作者：
KLINGLER, E
中科院分区：
文献类型：
--
作者：
ROBERTS, WE;MOZSARY, PG;KLINGLER, E
【大鼠】成骨前细胞的核形态测定是一种细胞动力学技术，适用于矿化组织的常规组织学准备。由于该方法是成骨前体细胞分化的形态学标记，为确定成骨细胞组织发生机制提供了一个新的维度。牙周韧带的成骨细胞前体是一个混合的祖细胞群体，根据核的大小可分为两个不同的群体。成骨前细胞是成骨细胞的直接增殖前体细胞，具有大细胞核（约170亩）。M3)，来源于相对未分化的成纤维细胞样细胞，其细胞核较小（< 80 .mu. M3）。细胞核体积的增加，在细胞周期的G1期，显然是基因组表达变化的形态学表现。这是成骨前细胞分化的关键事件，与机械应力/应变有关，可能是成骨细胞组织发生的重要限速步骤。
A nuclear morphometric assay for [rat] preosteoblasts is introduced as a cell-kinetic technique, applicable to routine histological preparations of mineralized tissue. Because this method is a morphological marker for osteoblast precursor cell differentiation, it provides a new dimension for determining the mechanism of osteoblast histogenesis. Osteoblast precursors of the periodontal ligament are a mixed population of progenitors, kinetically separable into 2 distinct groups according to nuclear size. Preosteoblasts, the immediate proliferating percursors of osteoblasts, have large nuclei (> 170 .mu.m3) and are derived from relatively undifferentiated fibroblastlike cells, which have smaller nuclei (< 80 .mu.m3). Increase in nuclear volume, during G1 phase of the cell cycle, is apparently a morphological manifestation of a change in genomic expression. This key event in preosteoblast differentiation is related to mechanical stress/strain and may be an important rate-limiting step in osteoblast histogenesis.