Heterogeneous nuclear ribonucleoprotein L interacts with the 3′ border of the internal ribosomal entry site of hepatitis C virus
Heterogeneous nuclear ribonucleoprotein L interacts with the 3′ border of the internal ribosomal entry site of hepatitis C virus
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DOI：
10.1128/jvi.72.11.8782-8788.1998
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发表时间：
1998-11-01
影响因子：
5.4
通讯作者：
Jang, SK
中科院分区：
文献类型：
--
作者：
Hahn, B;Kim, YK;Jang, SK
丙型肝炎病毒（HCV） RNA的翻译起始发生在核糖体以帽独立的方式进入5'非翻译区。尽管HCV内部核糖体进入位点（IRES）的精确边界尚未确定，但从大约核苷酸40 pip到coke蛋白编码序列N端的HCV RNA序列是有效的内部翻译起始所必需的。已经提出了几种细胞蛋白通过与HCV IRES结合来指导HCV IRES依赖的翻译。在这里，我们报道了一种新的细胞蛋白，它在核心编码序列中特异性地与HCV IRES的3'边界相互作用。该蛋白表观分子质量为68 kDa，为异质核核糖核蛋白L （hnRNP L）。hnRNP L与HCV IRES的结合与相应mrna的翻译效率相关。这一发现提示hnRNP L可能通过IRES元件在HCV mRNA的翻译中发挥重要作用。
Translation initiation of hepatitis C virus (HCV) RNA occurs by internal entry of a ribosome into the 5' nontranslated region in a cap-independent manner. The HCV RNA sequence from about nucleotide 40 pip to the N terminus of the coding sequence of the coke protein is required for efficient internal initiation of translation, though the precise border of the HCV internal ribosomal entry site (IRES) has yet to he determined. Several cellular proteins have been proposed to direct HCV IRES-dependent translation by binding to the HCV IRES. Here we report on a novel cellular protein that specifically interacts with the 3' border of the HCV IRES in the core-coding sequence. This protein with an apparent molecular mass of 68 kDa turned out to be heterogeneous nuclear ribonucleoprotein L (hnRNP L). The binding of hnRNP L to the HCV IRES correlates with the translational efficiencies of corresponding mRNAs. This finding suggests that hnRNP L may play an important role in the translation of HCV mRNA through the IRES element.