Rice OsAAA-ATPase1 is Induced during Blast Infection in a Salicylic Acid-Dependent Manner, and Promotes Blast Fungus Resistance
Rice OsAAA-ATPase1 is Induced during Blast Infection in a Salicylic Acid-Dependent Manner, and Promotes Blast Fungus Resistance
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DOI：
10.3390/ijms21041443
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发表时间：
2020-02-01
影响因子：
5.6
通讯作者：
Jiang, Chang-Jie
中科院分区：
文献类型：
--
作者：
Liu, Xinqiong;Inoue, Haruhiko;Jiang, Chang-Jie
脂肪酸（FAs）在植物防御反应中具有信号传导作用。我们先前报道了水稻OsSSI 2基因（编码硬脂酰酰基载体蛋白FA去饱和酶（SACPD））的突变或RNAi敲低（OsSSI 2-kd），显著增强了对稻瘟病菌Magnaporthe vulgaris和叶枯病菌Xanthomonasvulgaris pv的抗性。（Xoo）.转录组学分析鉴定了6个AAA-ATP酶家族基因（以下称为OsAAA-ATP酶1 -6）在OsSSI 2-kd植物中上调，以及其他已知的防御相关基因。本文对OsAAA-ATPase 1在水稻对M.米。在大肠杆菌中合成的OsAAA-ATPase 1具有ATP酶活性。OsAAA-ATPase 1的转录被水杨酸（SA）的功能类似物苯并噻二唑（BTH）诱导，而不被其他植物激素诱导。OsAAA-ATPase 1的转录也受到M.在SA依赖性的方式，作为基因诱导显着减弱在转基因水稻株系表达细菌基因（nahG）编码水杨酸羟化酶。OsAAA-ATPase 1的过表达显著增强了病程相关基因的表达，提高了对烟草黑胫病的抗性。相反，RNAi介导的对该基因的抑制损害了这种抗性。这些结果表明OsAAA-APTase 1在SA介导的抗稻瘟病菌M.米。
Fatty acids (FAs) have been implicated in signaling roles in plant defense responses. We previously reported that mutation or RNAi-knockdown (OsSSI2-kd) of the rice OsSSI2 gene, encoding a stearoyl acyl carrier protein FA desaturase (SACPD), remarkably enhanced resistance to blast fungus Magnaporthe oryzae and the leaf-blight bacterium Xanthomonas oryzae pv. oryzae (Xoo). Transcriptomic analysis identified six AAA-ATPase family genes (hereafter OsAAA-ATPase1-6) upregulated in the OsSSI2-kd plants, in addition to other well-known defense-related genes. Here, we report the functional analysis of OsAAA-ATPase1 in rice's defense response to M. oryzae. Recombinant OsAAA-ATPase1 synthesized in Escherichia coli showed ATPase activity. OsAAA-ATPase1 transcription was induced by exogenous treatment with a functional analogue of salicylic acid (SA), benzothiadiazole (BTH), but not by other plant hormones tested. The transcription of OsAAA-ATPase1 was also highly induced in response to M. oryzae infection in an SA-dependent manner, as gene induction was significantly attenuated in a transgenic rice line expressing a bacterial gene (nahG) encoding salicylate hydroxylase. Overexpression of OsAAA-ATPase1 significantly enhanced pathogenesis-related gene expression and the resistance to M. oryzae; conversely, RNAi-mediated suppression of this gene compromised this resistance. These results suggest that OsAAA-APTase1 plays an important role in SA-mediated defense responses against blast fungus M. oryzae.