Intracellular co-delivery of zinc ions and plasmid DNA for enhancing gene transfection activity
Intracellular co-delivery of zinc ions and plasmid DNA for enhancing gene transfection activity
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DOI：
10.1039/c3mt00226h
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发表时间：
2014-01-01
期刊：
影响因子：
3.4
通讯作者：
Kawakami, Hiroyoshi
中科院分区：
文献类型：
--
作者：
Asayama, Shoichiro;Matsuda, Kosho;Kawakami, Hiroyoshi
锌离子、甲基化聚（1-乙烯基咪唑）（PVIm-Me）和质粒DNA （pDNA）形成三元配合物进行基因传递。由此产生的Zn-PVIm-Me-pDNA复合物在细胞内传递Zn2+离子和pDNA，导致pDNA的核易位。利用含有核蛋白nf - κ B的pDNA结合序列，Zn2+离子和pDNA在细胞内共递送增强了基因表达。这些结果表明，由Zn-PVIm-Me-pDNA复合物传递的细胞内Zn2+离子激活了nf - κ B，增强了pDNA的核易位。综上所述，Zn-PVIm-Me-pDNA复合物能够通过PVIm-Me和共递送的细胞内Zn2+离子的协同作用增强基因转染活性。
Zinc ions, methylated poly(1-vinylimidazole) (PVIm-Me) and plasmid DNA (pDNA) have formed ternary complexes for gene delivery. The resulting Zn-PVIm-Me-pDNA complexes have delivered both Zn2+ ions and pDNA inside cells, leading to the nuclear translocation of the pDNA. By use of the pDNA containing a nuclear protein, NF-kappa B, binding sequence, the intracellular co-delivery of Zn2+ ions and pDNA has enhanced gene expression. These results suggest that the intracellular Zn2+ ions delivered by Zn-PVIm-Me-pDNA complexes activated the NF-kappa B, enhancing the nuclear translocation of the pDNA. In conclusion, it has been demonstrated that the Zn-PVIm-Me-pDNA complex is capable of enhancing the gene transfection activity by a synergic effect of the PVIm-Me and the co-delivered intracellular Zn2+ ions.