Molecular cloning and sequence analysis of the fusion glycoprotein gene of human parainfluenza virus type 2.
Molecular cloning and sequence analysis of the fusion glycoprotein gene of human parainfluenza virus type 2.
复制标题
人副流感病毒2型融合糖蛋白基因的分子克隆及序列分析。
DOI：
10.1016/0042-6822(90)90168-q
复制
发表时间：
1990
期刊：
影响因子：
3.7
通讯作者：
Ray,R
中科院分区：
文献类型：
--
作者：
Hu,XL;Compans,RW;Matsuoka,Y;Ray,R
通过与病毒RNA探针和衍生自其他副粘病毒融合蛋白基因中发现的保守序列的合成寡核苷酸杂交，将含有2.0 kb插入片段的cDNA克隆鉴定为人副流感病毒2型（PI 2）融合糖蛋白基因。糖蛋白基因的完整核苷酸序列通过双脱氧核苷酸测序程序测定，发现含有一个单一的大开放阅读框，编码551个氨基酸的蛋白质，计算分子量为59，664。P12融合蛋白与其他副粘病毒的融合蛋白的比较表明在全长、N-末端信号肽序列（氨基酸7至25）、C-末端跨膜区（氨基酸486至513）和在F 1亚基的N-末端的高度保守的融合序列区（氨基酸107至132）方面的相似性。
A cDNA clone containing a 2.0-kb insert was identified as the human parainfluenza virus type 2 (PI2) fusion glycoprotein gene by hybridizing with a viral RNA probe and a synthetic oligonucleotide derived from a conserved sequence found in other paramyxovirus fusion protein genes. The complete nucleotide sequence of the glycoprotein gene was determined by the dideoxynucleotide sequencing procedure and found to contain a single, large open reading frame encoding a protein of 551 amino acids with a calculated molecular weight of 59,664. Comparison of the P12 fusion protein with those of other paramyxoviruses indicated similarities in overall length, N-terminal signal peptide sequence (amino acids 7 to 25), C-terminal membrane-spanning region (amino acids 486 to 513), and a highly conserved fusion sequence region at the N-terminus of the F, subunit (amino acids 107 to 132).