Overproduction, purification, crystallization and preliminary X-ray diffraction analysis of YopM, an essential virulence factor extruded by the plague bacterium Yersinia pestis.
Overproduction, purification, crystallization and preliminary X-ray diffraction analysis of YopM, an essential virulence factor extruded by the plague bacterium Yersinia pestis.
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YopM 的过量生产、纯化、结晶和初步 X 射线衍射分析，YopM 是鼠疫细菌鼠疫耶尔森氏菌挤出的一种重要毒力因子。
DOI：
10.1107/s0907444900013378
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
Waugh,DS
中科院分区：
文献类型：
--
作者：
Evdokimov,AG;Anderson,DE;Routzahn,KM;Waugh,DS
A recombinant form of Yersinia pestis YopM with a C-terminal polyhistidine affinity tag has been overproduced in Escherichia coli, purified to homogeneity and crystallized using the hanging-drop vapor-diffusion technique. Several different crystal forms were obtained. The most suitable crystals for X-ray diffraction belonged to space groups P4222 (unit-cell parameters a = 109.36, b = 109.36, c = 101.50 Å) and C2221 (unit-cell parameters a = 71.73, b = 121.85, c = 189.79 Å). With a synchrotron-radiation source, these crystals diffracted to 2.4 and 1.9 Å resolution, respectively.
