Expression of neuroendocrine secretory protein 7B2 mRNA in the mouse and rat pituitary gland.
Expression of neuroendocrine secretory protein 7B2 mRNA in the mouse and rat pituitary gland.
复制标题
神经内分泌蛋白7B2 mRNA在小鼠和大鼠垂体中的表达。
作者：
M. Marcinkiewicz;P. Touraine;M. Mbikay;M. Chrétien
分泌多肽7B2在不同的内分泌和神经内分泌细胞中产生，被认为在激素分泌机制中发挥作用。在这项研究中，我们研究了7B2mRNA在小鼠和大鼠脑垂体中的表达模式。当用[35S]标记的反义cRNA探针进行原位杂交时，在前叶(促性腺激素细胞、甲状腺激素细胞、皮质激素细胞、生长激素细胞和催乳素细胞)和中间叶(黑素营养细胞)的几乎所有内分泌细胞中都检测到7B2mRNA的转录本。后叶为阴性。免疫细胞化学显示，7B2在中叶黑素细胞内、前叶促性腺激素细胞和甲状腺激素细胞内积聚。在培养条件下，研究了其他垂体腺细胞，如促肾上腺皮质激素细胞、促生长激素细胞和催乳素细胞产生7B2的问题。促肾上腺皮质激素细胞株ATT-20和促生长激素细胞株GH3均表达7B2mRNA，并含有放射免疫分析可检测到的7B2蛋白。然而，免疫细胞化学不能显示该蛋白。因此，7B2有可能在脑下垂体的所有激素合成细胞中产生，只储存在其中的一些细胞中，并在从其他细胞合成后迅速输出。
The secretory polypeptide 7B2 is produced in different endocrine and neuroendocrine cells, where it is presumed to play a role in the hormone secretion mechanism. In this study, we examined a pattern of 7B2 mRNA expression in the mouse and rat pituitary gland. When [35S]-labeled antisense cRNA probes were used for in situ hybridization, 7B2 mRNA transcripts were detected within virtually all endocrine cells of the anterior lobe (gonadotrophs, thyrotrophs, corticotrophs, somatotrophs and lactotrophs) and of the intermediate lobe (melanotrophs). The posterior lobe was negative. By immunocytochemistry, 7B2 accumulation was observed within melanotrophs in the intermediate lobe and within gonadotrophs and thyrotrophs in the anterior lobe. The question of 7B2 production in other pituitary cells, such as corticotrophs, somatotrophs and lactotrophs, was studied under culture conditions. The corticotroph AtT-20 and somatrotroph GH3 cell lines both expressed 7B2 mRNA and contained 7B2 protein detectable by radioimmunoassay. However, this protein could not be visualized by immunocytochemistry. Thus, it is possible that 7B2 is produced in all hormone-synthesizing cells of the pituitary gland, being stored only within some of them and rapidly exported after synthesis from others.