Intranasal challenge with B. pertussis leads to more severe disease manifestations in mice than aerosol challenge.
Intranasal challenge with B. pertussis leads to more severe disease manifestations in mice than aerosol challenge.
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DOI：
10.1371/journal.pone.0286925
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发表时间：
2023
期刊：
影响因子：
3.7
通讯作者：
中科院分区：
文献类型：
--
作者：
小鼠百日咳博德特氏菌攻击模型已用于临床前研究数十年。目前，全球研究人员采用的方法不一致，这使得比较研究结果变得困难。本研究的目的是利用CD-1小鼠模型，通过鼻内和气雾剂给药两种途径，了解通过每种途径引起的疾病表现的差异。我们观察到，两种途径的百日咳攻毒均导致呼吸道的剂量依赖性定植，但总体而言，鼻内攻毒导致鼻腔灌洗液、气管和肺部的细菌负荷增加。此外，与气溶胶刺激相比，高剂量鼻内刺激可诱导白细胞增多和促炎细胞因子反应。这些数据强调了在实验设计中应考虑的百日咳双歧杆菌攻击途径的关键差异。
The murine Bordetella pertussis challenge model has been utilized in preclinical research for decades. Currently, inconsistent methodologies are employed by researchers across the globe, making it difficult to compare findings. The objective of this work was to utilize the CD-1 mouse model with two routes of challenge, intranasal and aerosol administration of B. pertussis, to understand the differences in disease manifestation elicited via each route. We observed that both routes of B. pertussis challenge result in dose-dependent colonization of the respiratory tract, but overall, intranasal challenge led to higher bacterial burden in the nasal lavage, trachea, and lung. Furthermore, high dose intranasal challenge results in induction of leukocytosis and pro-inflammatory cytokine responses compared to aerosol challenge. These data highlight crucial differences in B. pertussis challenge routes that should be considered during experimental design.
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影响因子：
3.1
作者：
Boehm, Dylan T.;Hall, Jesse M.;Damron, F. Heath
通讯作者：
Damron, F. Heath
影响因子：
3.1
作者：
Mahon, BP;Ryan, MS;Mills, KHG
通讯作者：
Mills, KHG
影响因子：
3.7
作者：
Andreasen C;Powell DA;Carbonetti NH
通讯作者：
Carbonetti NH
影响因子：
--
作者：
Bitzer, Graham;Weaver, Kelly;Barbier, Mariette;Damron, F. Heath
通讯作者：
Damron, F. Heath
影响因子：
9.2
作者：
Boehm, Dylan T.;Wolf, M. Allison;Damron, F. Heath
通讯作者：
Damron, F. Heath