PRODUCTION, CHARACTERIZATION, AND APPLICATION OF MONOCLONAL-ANTIBODIES TO VIBRIO-CHOLERAE O139 SYNONYM BENGAL
PRODUCTION, CHARACTERIZATION, AND APPLICATION OF MONOCLONAL-ANTIBODIES TO VIBRIO-CHOLERAE O139 SYNONYM BENGAL
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DOI：
10.1128/cdli.1.1.51-54.1994
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发表时间：
1994-01-01
期刊：
影响因子：
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通讯作者：
ALBERT, MJ
中科院分区：
文献类型：
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作者：
QADRI, F;AZIM, T;ALBERT, MJ
小鼠单克隆抗体 (MAb) 是针对新发现的霍乱弧菌 0139 血清群经丙酮处理的全细胞而产生的，该血清群在印度和孟加拉国引起霍乱样疾病的流行。四种 MAb 特异性识别霍乱弧菌 0139 的脂多糖抗原。 Mab ICL9 和 ICL13 属于免疫球蛋白 M (IgM) 同种型，ICL11 属于 IgG3 同种型，ICL12 属于 Ig2b 同种型。 IgG2b 同种型的第五种 MAb ICL10 与霍乱弧菌 091 发生交叉反应。所有五种 MAb 在酶联免疫吸附测定、玻片凝集试验、运动抑制试验和间接免疫荧光试验中均识别霍乱弧菌 0139。在我们的临床实验室对这些单克隆抗体进行为期 1 个月的评估期间，所有 86 例通过兔多克隆抗血清诊断为霍乱弧菌 0139 的病例也均被这些单克隆抗体检测到，确立了它们作为高灵敏度和特异性诊断试剂的实用性。有了这些单克隆抗体，现在应该可以在流行性和地方性腹泻病例以及环境和食品样本中筛查霍乱弧菌 0139 血清群。
Mouse monoclonal antibodies (MAbs) were derived against acetone-treated whole cells of the newly recognized Vibrio cholerae 0139 serogroup which is causing epidemics of cholera-like disease in India and Bangladesh. Four MAbs specifically recognized the lipopolysaccharide antigens of V. cholera 0139. Mabs ICL9 and ICL13 were of the immunoglobulin M (IgM) isotype, ICL11 was of the IgG3 isotype, and ICL12 was of the Ig2b isotype. A fifth MAb, ICL10, of the IgG2b isotype cross-reacted with V. cholerae 091. All five MAbs recognized V. cholerae 0139 in an enzyme-linked immunosorbent assay, slide agglutination test, motility inhibition test, and indirect immunofluorescence test. During a 1-month evaluation of these MAbs in our clinical laboratory, all 86 cases diagnosed as V. cholerae 0139 by a rabbit polyclonal antiserum were also detected by these MAbs, establishing their utility as highly sensitive and specific diagnostic reagents. With these MAbs, it should now be possible to screen for the V. cholerae 0139 serogroup in epidemic and endemic diarrhea cases and in environmental and food samples.