In vivo Magnetic Resonance Imaging of Atherosclerotic Lesions with a Newly Developed Evans Blue-DTPA-Gadolinium Contrast Medium in Apolipoprotein-E-Deficient Mice
In vivo Magnetic Resonance Imaging of Atherosclerotic Lesions with a Newly Developed Evans Blue-DTPA-Gadolinium Contrast Medium in Apolipoprotein-E-Deficient Mice
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DOI：
10.1159/000109930
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发表时间：
2007-10
影响因子：
1.7
通讯作者：
S. Yasuda;Kenjiro Ikuta;T. Uwatoku;K. Oi;K. Abe;F. Hyodo;K. Yoshimitsu;Kohtaro Sugimura;Hideo Utsumi;Y. Katayama;H. Shimokawa
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文献类型：
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作者：
S. Yasuda;Kenjiro Ikuta;T. Uwatoku;K. Oi;K. Abe;F. Hyodo;K. Yoshimitsu;Kohtaro Sugimura;Hideo Utsumi;Y. Katayama;H. Shimokawa
背景资料：特异性检测动脉粥样硬化斑块的磁共振成像（MRI）造影剂可用于斑块的非侵入性检测。我们最近开发了一种新的造影剂，伊文思蓝-DTPA-钆（EB-DTPA-Gd），选择性地积累血管病变与内皮细胞的去除。在这项研究中，我们研究了EB-DTPA-Gd是否也适用于动脉粥样硬化斑块的体内成像。方法：我们使用不同年龄（3、6和12月龄）的雄性载脂蛋白E缺陷（ApoE-/-）小鼠和年龄匹配的雄性野生型小鼠。单次静脉注射EB-DTPA-Gd（160 µM/kg体重）后，在体内获得MRI T1信号。结果如下：静脉注射EB-DTPA-Gd后10-20 min内观察到主动脉壁信号强度增加，并维持30 min。ApoE-/-小鼠主动脉MRI增强随年龄增加而增加，而野生型小鼠未观察到这种增强。组织学检查表明，MRI增强部位与动脉粥样硬化斑块部位存在拓扑相关性。结论：EB-DTPA-Gd是一种有效的MRI造影剂，可用于动脉粥样硬化斑块的体内检测。
Background: Magnetic resonance imaging (MRI) contrast agents that specifically detect atherosclerotic plaque may be useful for the noninvasive detection of the plaque. We have recently developed a new contrast agent, Evans blue-DTPA-gadolinium (EB-DTPA-Gd), which selectively accumulates vascular lesions with endothelial removal. In this study, we examined whether EB-DTPA-Gd is also useful for in vivo imaging of atherosclerotic plaques. Methods: We used male apolipoprotein-E-deficient (ApoE–/–) mice of different ages (3, 6 and 12 months old) and age-matched male wild-type mice. After a single intravenous administration of EB-DTPA-Gd (160 µM/kg body weight), MRI T1 signal was obtained in vivo. Results: Increased signal intensity in the aortic wall was noted within 10–20 min after intravenous injection of EB-DTPA-Gd and was maintained for 30 min. The MRI enhancement in the aorta of ApoE–/– mice was increased in accordance with age, whereas no such enhancement was noted in wild-type mice. Histological examination demonstrated that there was a topological correlation between the site of MRI enhancement and that of atherosclerotic plaque. Conclusions: These results indicate that EB-DTPA-Gd is a useful MRI contrast medium for the in vivo detection of atherosclerotic plaques.