Increased Concentrations of Nerve Growth Factor and Brain-Derived Neurotrophic Factor in the Rat Cerebellum After Exposure to Environmental Enrichment
Increased Concentrations of Nerve Growth Factor and Brain-Derived Neurotrophic Factor in the Rat Cerebellum After Exposure to Environmental Enrichment
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DOI：
10.1007/s12311-009-0129-1
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发表时间：
2009-12-01
期刊：
影响因子：
3.5
通讯作者：
Petrosini, Laura
中科院分区：
文献类型：
--
作者：
Angelucci, Francesco;De Bartolo, Paola;Petrosini, Laura
环境富集（EE）对脑功能和解剖结构影响的分子机制部分归因于动物模型大脑皮层和海马中参与神经元存活和活动依赖性可塑性的蛋白质的上调，如神经生长因子（NGF）和脑源性神经营养因子（BDNF）。然而，目前，几乎没有迹象表明EE对小脑神经营养因子水平的影响。因此，在这项研究中，我们暴露于EE的雄性Wistar大鼠从断奶到5个月的年龄和评估的生产的神经生长因子和脑源性神经营养因子在小脑和比较的神经营养因子的变化，在这一地区与其他脑结构中获得的神经营养因子的生产或运输。我们发现，在大鼠暴露于EE从出生后第21天至第140天，一个显着增加的BDNF和NGF的浓度在小脑中观察到，相比，在标准条件下饲养的大鼠。此外，小脑是脑区域，其中NGF和BDNF水平受EE的影响比在其他区域观察到的变化更大。EE还引起纹状体中NGF水平的伴随增加，而在同一脑区中，BDNF水平降低。总之，这项研究表明，长期暴露于EE与小脑NGF和BDNF的产生增加，从而表明EE对成年动物小脑的有益作用可以介导，至少部分，由神经营养因子。
The molecular mechanism of environmental enrichment (EE) on brain function and anatomy has been partially attributed to the up-regulation of proteins involved in neuronal survival and activity-dependent plasticity, such as the neurotrophins nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF), in the cerebral cortex and hippocampus of animal models. Nevertheless, at present, little indication is available on the influence of EE on neurotrophin levels in the cerebellum. Thus, in this study, we exposed male Wistar rats to EE from weaning to 5 months of age and evaluated the production of NGF and BDNF in the cerebellum and compared the neurotrophin changes in this region with those obtained in other brain structures where neurotrophins are produced or transported. We found that in rats exposed to EE from 21st until 140th postnatal day, a significant increase of both BDNF and NGF concentrations was observed in the cerebellum, as compared to rats reared in standard conditions. In addition, cerebellum was the brain region where NGF and BDNF levels were more influenced by EE as compared to the changes observed in other regions. EE also caused a concomitant increase in NGF levels in the striatum while in the same brain region, BDNF levels were reduced. In summary, this study shows that a prolonged exposure to EE is associated with an increase in cerebellar NGF and BDNF production, thus suggesting that the beneficial effects of EE on the cerebellum of adult animals could be mediated, at least in part, by neurotrophins.