Cutaneous lymphocyte antigen and α4β7 T-lymphocyte responses are associated with peanut allergy and tolerance in children
Cutaneous lymphocyte antigen and α4β7 T-lymphocyte responses are associated with peanut allergy and tolerance in children
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DOI：
10.1111/j.1398-9995.2011.02765.x
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发表时间：
2012-03-01
期刊：
影响因子：
12.4
通讯作者：
Lack, G.
中科院分区：
文献类型：
--
作者：
Chan, S. M. H.;Turcanu, V.;Lack, G.
背景资料：目前尚不清楚早期过敏原暴露的初始途径是否会影响过敏的后续发展，皮肤致敏导致花生过敏（PA），以及口服暴露诱导的耐受性。皮肤和胃肠道（GI）归巢标志物，皮肤淋巴细胞抗原（CLA）和α 4 β 7整合素，用于确定PA的状态是否与花生特异性CLA反应相关，与主要的α 4 β 7 responses.Methods相关的耐受性：CLA+和α 4 β 7+记忆T细胞分离和培养与花生提取物，以评估其增殖。刺激指数在花生过敏和非过敏（NA）组进行了比较，花生特异性细胞因子的生产measured.Results：在花生过敏患者，花生特异性增殖占主导地位的皮肤归巢CLA+子集，而花生耐受组有混合的CLA/a4 b7反应（P = 0.008）。与对照食物抗原（卵清蛋白）的比较表明，这些差异是过敏原特异性的。细胞因子反应呈Th 1偏斜的趋势，在GI-归巢的a4 b7+细胞的花生耐受组和Th 2偏斜的皮肤归巢的CLA+细胞的花生allergics.Conclusion：花生过敏患者的CLA+响应花生的优势是一致的假设，过敏性致敏发生通过皮肤。花生耐受组中主要的α 4 β 7+反应表明，通过胃肠道接触过敏原可诱导耐受性。
Background: It is unclear whether the initial route of allergen exposure in early life could influence the subsequent development of allergy, with cutaneous sensitization leading to peanut allergy (PA), and tolerance induced by oral exposure. The skin-and gastrointestinal (GI)-homing markers, cutaneous lymphocyte antigen (CLA) and alpha 4 beta 7 integrin, are used to determine whether the state of PA correlates with peanut-specific CLA responses, with tolerance associated with predominant alpha 4 beta 7 responses.Methods: CLA+ and alpha 4 beta 7+ memory T cells were isolated and cultured with peanut extract to assess their proliferation. Stimulation indices were compared in peanut allergic and non-allergic (NA) groups, and peanut-specific cytokine production was measured.Results: In peanut allergic patients, peanut-specific proliferation predominates in the skin-homing CLA+ subset, whilst peanut-tolerant groups have a mixed CLA/ a4b7 response (P = 0.008). Comparison with a control food antigen (ovalbumin) showed that these differences are allergen specific. Cytokine responses showed trends towards Th1 skewing in the GI-homing a4b7+ cells of peanut-tolerant groups and Th2 skewing in the skin-homing CLA+ cells of peanut allergic patients.Conclusion: The predominance of the CLA+ response to peanut in peanut allergic patients is consistent with the hypothesis that allergic sensitization occurs through the skin. The predominant alpha 4 beta 7+ response in peanut-tolerant groups suggests that allergen exposure through the GI tract induces tolerance.