Drosophila ventral furrow morphogenesis:: a proteomic analysis
Drosophila ventral furrow morphogenesis:: a proteomic analysis
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DOI：
10.1242/dev.00955
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发表时间：
2004-02-01
期刊：
影响因子：
4.6
通讯作者：
Minden, JS
中科院分区：
文献类型：
--
作者：
Gong, L;Puri, M;Minden, JS
果蝇原肠胚形成过程中，中胚层前体的内化是一个关键的形态发生事件。虽然遗传分析已经揭示了参与腹沟细胞特化的基因，但很少有作为腹沟细胞行为介质的结构蛋白被鉴定出来。使用差异凝胶电泳的比较蛋白质组学方法来鉴定腹侧胚胎与侧侧胚胎中丰度水平或亚型变化的五十多种蛋白质。奇怪的是，这些胚胎之间的大多数蛋白质差异早在原肠胚形成之前就出现了，只有少数蛋白质变化与原肠胚形成一致，这表明腹侧细胞为细胞形状变化做好了准备。三个蛋白酶体亚基被发现腹侧化和侧化胚胎之间的差异。RNAi敲低这些蛋白酶体亚基和时间依赖性差异蛋白导致腹沟缺陷，验证了这些蛋白在腹沟形态发生中的作用。
Ventral furrow formation is a key morphogenetic event during Drosophila gastrulation that leads to the internalization of mesodermal precursors. While genetic analysis has revealed the genes involved in the specification of ventral furrow cells, few of the structural proteins that act as mediators of ventral cell behavior have been identified. A comparative proteomics approach employing difference gel electrophoresis was used to identify more than fifty proteins with altered abundance levels or isoform changes in ventralized versus lateralized embryos. Curiously, the majority of protein differences between these embryos appeared well before gastrulation, only a few protein changes coincided with gastrulation, suggesting that the ventral cells are primed for cell shape change. Three proteasome subunits were found to differ between ventralized and lateralized embryos. RNAi knockdown of these proteasome subunits and time-dependent difference-proteins caused ventral furrow defects, validating the role of these proteins in ventral furrow morphogenesis.