Comparison of a DNA based PCR method with conventional methods for the detection of M. tuberculosis in Jos, Nigeria
Comparison of a DNA based PCR method with conventional methods for the detection of M. tuberculosis in Jos, Nigeria
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DOI：
10.3855/jidc.420
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发表时间：
2009-07-01
影响因子：
1.9
通讯作者：
Idoko, John
中科院分区：
文献类型：
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作者：
Ani, Agatha;Okpe, Silvanis;Idoko, John
背景资料：为实现肺结核的早期诊断和有效治疗，提高结核分枝杆菌（M。结核病）。本研究比较了基于插入序列（IS 6110）的聚合酶链反应（PCR）检测方法与传统方法检测M。在资源有限的情况下从临床标本中分离结核病。方法：来自101名艾滋病毒阳性患者和40份临床标本的痰液（腹水、胃冲洗液、腹水、胸膜液和脑脊液）（HIV状态未知），均在尼日利亚乔斯的乔斯大学教学医院（JUTH）疑似肺结核，通过Ziehl Neelsen（ZN）涂片显微镜检查，Lowenstein詹森（LJ）的卵基培养和PCR方法检测M.结核病结果：HIV阳性患者标本中45/101（44.6%）检出分枝杆菌，其中ZN（+）培养（+）PCR（+）6%，ZN（-）培养（+）PCR（-）4%，ZN-培养（+）PCR（+）16%，ZN（-）培养（-）PCR（+）19%。40名儿童中有22名（55%）为阳性，涂片镜检率为0%; 4/40（10%）为培养（+）PCR（+）; 18/40（45%）为培养（-）PCR（+）。PCR对HIV阳性患者的敏感性和特异性分别为85%和74%，而ZN涂片镜检的敏感性和特异性分别为23%和100%。结论：IS 6110 PCR是一种快速、敏感、特异的方法。结核病综合征组。该方法操作简单，提高了对M.结核病从检查的标本。我们建议将其用于M.在结核病和艾滋病毒高负担地区，
Background: To achieve early diagnosis and effective treatment of pulmonary tuberculosis, simple and sensitive methods that enhance the detection of Mycobacterium tuberculosis (M. tuberculosis) from clinical specimens are needed. This study compared the effectiveness and suitability of an insertion sequence (IS 6110) based polymerase chain reaction (PCR) assay with conventional methods for the detection of M. tuberculosis from clinical specimens in a resource-limited setting.Methods: Sputa from 101 HIV-positive patients and 40 clinical specimens (sputa, gastic wash out, ascitic fluid, pleural fluid and cerebrospinal fluid) collected from children (HIV status unknown), all suspected for pulmonary tuberculosis at the Jos University Teaching Hospital, Jos, (JUTH) Nigeria, were examined by Ziehl Neelsen (ZN) smear microscopy, Lowenstein Jensen's (LJ) egg-based culture, and PCR methods for the detection of M. tuberculosisResults: Mycobacteria was detected in 45/101 (44.6%) of the specimens from the HIV-positive patients and comprised of 6% ZN(+) culture(+)PCR(+), 4% ZN(-)culture(+)PCR(-), 16% ZN-culture(+)PCR(+) and 19% ZN(-)culture(-)PCR(+). Twenty-two of forty (55%) children were positive with 0% smear microscopy; 4/40 (10%) culture(+)PCR(+); and 18/40 (45%) culture(-)PCR(+). The sensitivity and specificity of the PCR for the HIV-positive patients were 85% and 74% respectively against 23% and 100% for ZN smear microscopy.Conclusion: The IS6110 PCR is a rapid and sensitive method that is specific for the M. tuberculosis complex group. It is simple in our experience and increased the detection of M. tuberculosis from the specimens examined. We suggest its use for the detection of M. tuberculosis in high TB and HIV burden areas.