Increased production of soluble inducible costimulator in patients with diffuse cutaneous systemic sclerosis
Increased production of soluble inducible costimulator in patients with diffuse cutaneous systemic sclerosis
复制标题
DOI：
10.1007/s00403-012-1292-7
复制
发表时间：
2013-01-01
影响因子：
3
通讯作者：
Sato, Shinichi
中科院分区：
文献类型：
--
作者：
Yanaba, Koichi;Asano, Yoshihide;Sato, Shinichi
诱导型共刺激分子（ICOS）对于T细胞增殖、各种细胞因子的产生和T细胞依赖性B细胞应答至关重要。为探讨系统性硬化症（SSc）患者血清可溶性ICOS（sICOS）水平及其与临床指标的关系，采用酶联免疫吸附法检测了38例SSc患者和24例健康人血清sICOS水平。免疫组化法检测ICOS及其配体在皮肤中的表达。SSc患者血清sICOS水平与健康对照组无显著性差异。弥漫型SSc患者sICOS水平高于局限型SSc患者（P < 0.05）和健康人（P < 0.05）。血清sICOS水平与皮肤硬化程度呈正相关。与sICOS水平正常的患者相比，SSc和sICOS水平升高的患者更容易发生间质性肺疾病和肺活量下降。早期SSc患者血清sICOS水平显著高于晚期SSc患者。ICOS和ICOS配体免疫组化染色可见于皮损组织中浸润的真皮单个核细胞。这些结果表明，sICOS的血清水平在弥漫性皮肤SSc患者中升高，并与皮肤硬化和间质性肺病的严重程度和活动性相关。ICOS可能有助于SSc的发展。此外，检测早期SSc患者血清sICOS水平可为进一步评估SSc疾病严重程度提供重要手段。
Inducible costimulator (ICOS) is crucial for T cell proliferation, production of various cytokines, and T cell-dependent B-cell responses. To determine the serum soluble ICOS (sICOS) level and its association with clinical parameters in patients with systemic sclerosis (SSc), serum sICOS level was examined by enzyme-linked immunosorbent assay in 38 patients with SSc and 24 healthy individuals. The expression of ICOS and ICOS ligand in skin was examined immunohistochemically. There was no significant difference in serum sICOS level between patients with SSc and healthy individuals. Patients with diffuse cutaneous SSc had higher levels of sICOS than those with limited cutaneous SSc (P < 0.05) or healthy individuals (P < 0.05). Serum sICOS level correlated positively with the severity of skin sclerosis. Patients with SSc and elevated sICOS level more often had interstitial lung disease and decreased vital capacity than those with normal sICOS level. The serum sICOS level was significantly greater in patients with early phase SSc than those with late phase SSc. ICOS and ICOS ligand immunostaining were observed on infiltrating dermal mononuclear cells in lesional skin tissue. These results suggest that the serum level of sICOS is increased in patients with diffuse cutaneous SSc and correlates with the severity and activity of skin sclerosis and interstitial lung disease. ICOS may contribute to the development of SSc. In addition, measurement of serum sICOS level in patients with early SSc may offer an important means for further evaluation of SSc disease severity.