Deletions on mouse Yq lead to upregulation of multiple X- and Y-linked transcripts in spermatids
Deletions on mouse Yq lead to upregulation of multiple X- and Y-linked transcripts in spermatids
复制标题
DOI：
10.1093/hmg/ddi304
复制
发表时间：
2005-09-15
影响因子：
3.5
通讯作者：
Burgoyne, PS
中科院分区：
文献类型：
--
作者：
Ellis, PJI;Clemente, EJ;Burgoyne, PS
小鼠Y染色体长臂（MSYq）的缺失会导致畸形精子症，严重时会导致不育。我们发现，在睾丸中的下游转录变化造成的损失MSYq编码的转录涉及多个X-和Y-连锁精子表达基因的上调，但不相关的常染色体基因。因此，这表明在正常男性中，在减数分裂后细胞中存在对X和Y（生殖体）转录的特异性抑制，这取决于MSYq编码的转录物。连同已知的性别比例偏向有利于女性在生育的MSYqdel男性的后代，这强烈表明存在的X和Y连锁基因之间的基因组内冲突。两个潜在的拮抗剂在这一冲突是X连锁的多拷贝基因Xmr和其多拷贝MSYq连锁的相对Sly，这是上调和下调，分别在睾丸中的MSYqdel男性。Xmr也在减数分裂性染色体失活（MSCI）期间表达，表明MSCI与精子细胞中MSYq依赖性染色体抑制之间存在联系。因此，我们提出，这种抑制和MSCI本身是进化适应，以维持正常的性别比例，在面对X/Y拮抗。
Deletions on the mouse Y-chromosome long arm (MSYq) lead to teratozoospermia and in severe cases to infertility. We find that the downstream transcriptional changes in the testis resulting from the loss of MSYq-encoded transcripts involve upregulation of multiple X- and Y-linked spermatid-expressed genes, but not related autosomal genes. Therefore, this indicates that in normal males, there is a specific repression of X and Y (gonosomal) transcription in post-meiotic cells, which depends on MSYq-encoded transcripts. Together with the known sex ratio skew in favour of females in the offspring of fertile MSYqdel males, this strongly suggests the existence of an intragenomic conflict between X- and Y-linked genes. Two potential antagonists in this conflict are the X-linked multicopy gene Xmr and its multicopy MSYq-linked relative Sly, which are upregulated and downregulated, respectively, in the testes of MSYqdel males. Xmr is also expressed during meiotic sex chromosome inactivation (MSCI), indicating a link between the MSCI and the MSYq-dependent gonosomal repression in spermatids. We therefore propose that this repression and MSCI itself are evolutionary adaptations to maintain a normal sex ratio in the face of X/Y antagonism.