A Subtle Change in p38 MAPK Activity is Sufficient to Suppress In Vivo Tumorigenesis
A Subtle Change in p38 MAPK Activity is Sufficient to Suppress In Vivo Tumorigenesis
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DOI：
10.4161/cc.4.1.1342
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发表时间：
2005-01
期刊：
影响因子：
4.3
通讯作者：
O. Timofeev;Ting Yi Lee;D. Bulavin
中科院分区：
文献类型：
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作者：
O. Timofeev;Ting Yi Lee;D. Bulavin
新出现的证据支持p38 MAPK在肿瘤发生中的负调控作用。在这里，我们展示了p38 MAPK的微妙激活足以抑制肿瘤发生，通过将mkk6诱导的细胞外植到裸鼠体内形成肿瘤的能力来测量。另一方面，p38 MAPK的激活并不一定会立即引起体外细胞生长的抑制，这是通过标准MTS试验测量的。这一数据揭示了一种新的抗癌药物筛选方法，并表明大量潜在的抗肿瘤化合物，如MKK6/p38信号激活剂，在以前基于常规生长抑制试验的高通量筛选中被遗漏。
Emerging evidence supports a role for p38 MAPK in negative regulation of tumorigenesis. Here we show that a subtle activation of p38 MAPK is sufficient to suppress tumorigenesis as measured by the ability to form tumors when MKK6-inducible cells were explanted into nude mice. On the other hand, this activation of p38 MAPK did not necessarily cause an immediate inhibition of cell growth in vitro as measured by standard MTS assay. This data uncovers a new methodology for anti-cancer drugs screening and suggests that a substantial number of potential anti-tumor compounds, such as activators of MKK6/p38 signaling, was missed out in previous high throughput screens based on conventional growth inhibition assays.