Chimeric Maternal Cells with Tissue-Specific Antigen Expression and Morphology Are Common in Infant Tissues
Chimeric Maternal Cells with Tissue-Specific Antigen Expression and Morphology Are Common in Infant Tissues
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DOI：
10.2350/08-07-0499.1
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发表时间：
2009-09-01
影响因子：
1.9
通讯作者：
Nelson, J. Lee
中科院分区：
文献类型：
--
作者：
Stevens, Anne M.;Hermes, Heidi M.;Nelson, J. Lee
母体微嵌合体（MMc）被认为在自身免疫的发病机制中发挥作用，但少量外源细胞如何导致慢性全身性炎症尚未得到解释。外周血细胞分化为组织特异性细胞类型的报道可能会阐明嵌合母体细胞可以作为组织内靶细胞的问题。我们调查了7名男婴的组织中的MMc。女性细胞，假定母亲，其特征在于同时免疫组织化学和荧光原位杂交的X和Y染色体母细胞构成的实质细胞的0.017%至1.9%，并发现在所有婴儿的肝脏，胰腺，肺，肾，膀胱，皮肤和脾脏。分化母体细胞：肝脏中的母体肝细胞、肾脏中的肾小管细胞和胰腺中的β-胰岛细胞。在组织损伤或炎症浸润区域未发现母体细胞。母体造血细胞仅在新生儿狼疮患者的心脏中发现。因此，分化的母体细胞存在于多种组织类型中，并且独立于炎症或组织损伤而发生。对母体实质细胞的耐受性丧失可能导致器官特异性“自身”炎症性疾病和炎症区域中母体细胞的消除。
Maternal microchimerism (MMc) has been purported to play a role in the pathogenesis of autoimmunity, but how a small number of foreign cells could contribute to chronic, systemic inflammation has not been explained. Reports of peripheral blood cells differentiating into tissue-specific cell types may shed light on the problem in that chimeric maternal cells could act as target cells within tissues. We investigated MMc in tissues from 7 male infants. Female cells, presumed maternal, were characterized by simultaneous immunohistochemistry and fluorescence in situ hybridization for X- and Y-chromosomes Maternal cells constituted 0.017% to 1.9% of parenchymal cells and were found in all infants in liver, pancreas, lung, kidney, bladder, skin, and spleen. Maternal cells were differentiated: maternal hepatocytes in liver, renal tubular cells in kidney, and beta-islet cells in pancreas. Maternal cells were not found in areas of tissue injury or inflammatory infiltrate. Maternal hematopoietic cells were found only in hearts from patients with neonatal lupus. Thus, differentiated maternal cells are present in multiple tissue types and occur independently of inflammation or tissue injury. Loss of tolerance to maternal parenchymal cells could lead to organ-specific "auto" inflammatory disease and elimination of maternal cells in areas of inflammation.