Isolation and characterization of the RanGAP gene in the mosquito Aedes aegypti.
Isolation and characterization of the RanGAP gene in the mosquito Aedes aegypti.
复制标题
埃及伊蚊 RanGAP 基因的分离和表征。
DOI：
10.1080/10425170600805540
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发表时间：
2006
期刊：
影响因子：
--
通讯作者：
Severson,DavidW
中科院分区：
文献类型：
--
作者：
Cha,Sung-Jae;Lobo,Neil;Debruyn,Becky;Severson,DavidW
A duplicated 3′-truncated version ofRanGAPwas previously identified asSegregation distorter(Sd), the meiotic drive gene inDrosophila melanogaster. Here we report the cloning and characterization of the complete gene sequence for theRanGAPhomolog from the mosquitoAedes aegypti. The 1995 bp cDNA sequence consists of a 113 bp 5′ UTR and 130 bp 3′ UTR, and encodes a 583 amino acid protein with high sequence identity with RanGAP homologues of several species. A 20,125 bp genomic DNA sequence contains the completeRanGAPgene, consisting of three exons and two introns. Intron 2 comprises 18,082 bp and contains multiple repetitive elements as well as putative coding regions. TheRanGAPlocus was mapped to the q-arm of chromosome 2. Because the meiotic drive gene (MD) inA. aegyptiwas previously shown to be tightly linked with the sex determining locus on chromosome 1,RanGAPis likely not theMDgene.
