Coagulation factor XIII-A -: A flow cytometric intracellular marker in the classification of acute myeloid leukemias
Coagulation factor XIII-A -: A flow cytometric intracellular marker in the classification of acute myeloid leukemias
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DOI：
10.1160/th05-03-0206
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发表时间：
2005-08-01
影响因子：
6.7
通讯作者：
Muszbek, L
中科院分区：
文献类型：
--
作者：
Kappelmayer, J;Simon, A;Muszbek, L
凝血因子与白细胞的相关性已在先前的几项研究中得到证实。本研究旨在研究培养的成髓细胞和单核细胞系中因子XIII亚基A（FXIII-A）标记的敏感性和特异性，并研究新生急性髓性白血病（AML）样本中FXIII-A的胞浆内表达。培养成髓细胞和单核细胞系，并研究谱系特异性成熟标志物和FXIII-A表达。此外，在12个正常样本（7个骨髓和5个外周血）、86个原发AML样本和6个慢性粒单核细胞白血病（CMML）样本中研究FXIII-A表达。在单核细胞MonoMac6细胞系中，FXIII-A的出现先于CID 14的出现，而在成髓细胞PLB-985细胞系中，FXIII-A在其整个成熟期内保持阴性。在AML中，FXIII-A阳性细胞在髓系白血病中的平均频率低于10%，而在M4和MSAML中的平均频率高于50%，且显著高于常用的CD 14标记物（p <0.0001）。在AML M4和M5病例中，FXIII-A被证明对单核母细胞的鉴定敏感。FXIII-A可被认为是单核细胞和巨核细胞系列的可靠胞浆内标志物，其存在高度预测单核细胞和巨核细胞AML以及CMML。
The association of coagulation factors with leukocytes have been demonstrated in several previous studies. This study was designed to study the sensitivity and specificity of factor XIII subunit A (FXIII-A) labelling in cultured myeloblastic and monoblastic cell lines and to investigate the intracytoplasmic expression of FXIII-A in de novo acute myeloid leukemia (AML) samples. Myeloblastic and a monoblastic cell lines were cultured and investigated for lineage specific maturation markers and FXIII-A expression. Furthermore, FXIII-A expression was investigated in 12 normal samples (7 bone marrow and 5 peripheral blood), 86 de novo AML samples and 6 chronic myelomonocytic leukemia (CMML) samples. In the monoblastic MonoMac6 cell line the appearance of FXIII-A preceded that of CID 14 while it remained negative in the myeloblastic PLB-985 cell line throughout its maturation period. Among the AML samples the average frequency of FXIII-A positive cells in myeloblastic leukemia samples was below 10%,while in M4 and MSAML samples it was above 50% and was significantly higher than the generally used CD 14 marker (p < 0.0001). In the AML M4 and M5 cases, FXIII-A proved sensitive for the identification of monoblasts. FXIII-A can be considered as a reliable intracytoplasmic marker for the monocytic and megakaryocytic series and its presence is highly predictive for mono- and megakaryocytic AML and for CMML.