Regenerative organization of the trigeminal ganglion following mental nerve section and repair in the adult rat.
Regenerative organization of the trigeminal ganglion following mental nerve section and repair in the adult rat.
复制标题
成年大鼠颏神经切断和修复后三叉神经节的再生组织。
DOI：
10.1002/cne.902950404
复制
发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Hegtvedt,AK
中科院分区：
文献类型：
--
作者：
Zuniga,JR;Pate,JD;Hegtvedt,AK
采用连续双荧光标记技术确定成年大鼠颏神经切断和手术修复后一级下颌神经元的再生体视组织。在以下双标记范例中对来自12只成年大鼠的24个神经节进行显微镜检查：（i）将固蓝直接注射到双侧颏神经中;（ii）7天后，切断神经并立即通过显微镜缝合技术重新连接;（iii）在修复后30、60和90天，将二脒基黄直接注射到再生神经中，远离修复点;和（iv）3天后处死动物，取出神经节进行荧光显微镜检查。结果与未修复/切除对照组和假手术对照组的12个神经节进行了比较，所有组中荧光标记的下颌骨细胞的组织结构均遵循沿着三叉神经节的外侧背腹轴的有序体视分布。组内和组间荧光标记细胞的平均总数差异不显著或极小。没有证据表明神经元再生后有异源（非下颌）或同源（下颌）发芽。如果横断损伤未修复，则通过双标记细胞的存在确定的再生可忽略不计，但在修复后30天显著。此外，下颌骨再生逐渐改善，如修复后60和90天双标记的显著增加所示。这些研究结果表明，成年大鼠神经切断和立即修复后：（i）精神感觉神经元胞体从三叉神经节下颌部再生并保持一个有组织的躯体定位区;（ii）成年大鼠非颏感觉下颌和/或非下颌三叉神经节细胞的侧支芽重组不明显或可忽略不计;和（iii）切除的三叉神经感觉神经元的再生是一个渐进的过程，其通过立即手术干预而增强。
Sequential double‐fluorescence labeling techniques were employed to determine the regenerative somatotopic organization of first‐order mandibular neurons following mental nerve transection and surgical repair in the adult rat. Twenty‐four ganglia from 12 adult rats were examined microscopically in the following double‐labeling paradigm: (i) Fast Blue was injected directly into the mental nerves bilaterally; (ii) 7 days later the nerves were transected and immediately rejoined by microscopic suture techniques; (iii) Diamidino Yellow was then injected directly into the regenerated nerve, distal to the point of repair, 30, 60, and 90 days postrepair; and (iv) the animals were sacrificed 3 days later and the ganglia removed for fluorescent microscopic examination. Results were compared with 12 ganglia each of unrepaired/resected controls and sham surgery controls made in parallel.The organization of fluorescence‐labeled mandibular cells followed an orderly somatotopic distribution along the lateral dorsoventral axis of the trigeminal ganglion in all groups. The difference in mean total number of fluorescence‐labeled cells within and between groups was insignificant or minimal. There was no evidence of heteronymous (nonmandibular) or homonymous (mandibular) sprouting following neuronal regeneration. Regeneration, as determined by the presence of double‐labeled cells, was negligible if the transection injury was not repaired but significant 30 days following repair. Additionally, mandibular regeneration gradually improved, as shown by the significant increase of double‐labeling at 60 and 90 days postrepair. However, 90 days later, the percentage of regenerated cells had not reached sham control conditions.The results of these studies suggest that following nerve transection and immediate repair in the adult rat: (i) mental sensory neuronal perikarya regenerate from and maintain an organized somatotopic area within the mandibular division of the trigeminal ganglion; (ii) reorganization by collateral sprouts from nonmental sensory mandibular and/or nonmandibular trigeminal ganglion cells is not evident or is negligible in the adult rat; and (iii) regeneration of resected trigeminal sensory neurons is a gradual process which is enhanced by immediate surgical intevention.