FINE-STRUCTURE OF INFLUENZA-A VIRUS OBSERVED BY ELECTRON CRYOMICROSCOPY
FINE-STRUCTURE OF INFLUENZA-A VIRUS OBSERVED BY ELECTRON CRYOMICROSCOPY
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DOI：
10.1002/j.1460-2075.1994.tb06264.x
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发表时间：
1994-01-15
期刊：
影响因子：
11.4
通讯作者：
SATO, SB
中科院分区：
文献类型：
--
作者：
FUJIYOSHI, Y;KUME, NP;SATO, SB
用高分辨率电子冷冻显微镜观察快速冷冻的甲型流感病毒玻璃化水悬浮液。流感病毒颗粒分为内部组织良好的小球形颗粒（直径< 150nm）、内部组织较差的大球形颗粒和丝状颗粒。大多数病毒大颗粒的包膜是磷脂双层，含有这些大颗粒的层析部分在很大程度上缺乏病毒活性。另一方面，大多数丝状和小球形病毒颗粒的包膜形成了一种奇怪的对比，这可以归因于薄的外层脂质单层和7.2纳米厚的含有蛋白质的内层的结合。这些后一种颗粒代表了制备过程中大部分的病毒活性。近焦图像的密度测量痕迹证实了这些结构差异。一些病毒包膜结构显然介于这两种不同类型的膜之间。根据这些结果，结合计算机模拟，建立了完整的具有生物活性的流感病毒颗粒的结构模型。
A rapidly frozen vitrified aqueous suspension of influenza A virus was observed by high resolution electron cryomicroscopy. The influenza particles were grouped into small (diameter < 150 nm) spherical particles with well organized interiors, large spherical ones with less internal organization, and filamentous ones. Envelopes of most of the large virus particles were phospholipid bilayers, and the chromatography fraction containing these large particles was largely devoid of viral activity. The envelopes of most of the filamentous and small spherical virus particles, on the other hand, gave a strange contrast which could be ascribed to a combination of a thin outer lipid monolayer and a 7.2 nm thick protein-containing inner layer. These latter particles represented most of the viral activity in the preparation. Densitometric traces of the near in-focus images confirmed these structural differences. Some viral envelope structures apparently intermediate between these two distinct types of membrane were also detected. A structural model of intact biologically active influenza virus particles was formulated from these results, together with computer simulations.