Involvement of Arabidopsis histone deacetylase HDA6 in ABA and salt stress response
Involvement of Arabidopsis histone deacetylase HDA6 in ABA and salt stress response
复制标题
拟南芥组蛋白脱乙酰酶 HDA6 参与 ABA 和盐胁迫反应
DOI：
10.1093/jxb/erq154
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发表时间：
2010-07-01
影响因子：
6.9
通讯作者：
Wu, Keqiang
中科院分区：
文献类型：
--
作者：
Chen, Li-Ting;Luo, Ming;Wu, Keqiang
组蛋白修饰在基因表达的表观遗传调节中发挥着重要作用。所有的组蛋白修饰都是可逆的，因此可以提供一种灵活的方式来调节植物发育期间和植物对环境刺激的反应期间的基因表达。核心组蛋白上特定赖氨酸残基的可逆乙酰化和脱乙酰化由组蛋白乙酰转移酶和组蛋白脱乙酰酶（HDAs）催化。HDA 6是拟南芥中的RPD 3型组蛋白脱乙酰酶。拟南芥HDA 6突变体，axe 1 -5，和HDA 6 RNA干扰植物表现出的表型，是阿坝和盐胁迫超敏感。与野生型植物相比，阿坝和非生物胁迫响应基因ABI 1，ABI 2，KAT 1，KAT 2，DREB 2A，RD 29 A和RD 29 B在axe 1 -5和HDA 6 RNA干扰植物中的表达在阿坝或盐胁迫处理时降低。结果表明，阿坝和盐胁迫均能使阿坝和非生物胁迫响应基因的组蛋白H3 K9 K14乙酰化和H3 K4三甲基化基因激活标记富集，而抑制标记H3 K9二甲基化基因减少。我们的研究表明，HDA 6参与组蛋白修饰调节种子萌发和盐胁迫反应，以及阿坝和盐胁迫诱导的基因表达在拟南芥。
Histone modifications play an important role in the epigenetic regulation of gene expression. All histone modifications are reversible, which may therefore provide a flexible way for regulating gene expression during the plant's development and during the plant response to environmental stimuli. The reversible acetylation and deacetylation of specific lysine residues on core histones are catalysed by histone acetyltransferases and histone deacetylases (HDAs). HDA6 is an RPD3-type histone deacetylase in Arabidopsis. The Arabidopsis HDA6 mutant, axe1-5, and HDA6 RNA-interfering plants displayed a phenotype that was hypersensitive to ABA and salt stress. Compared with wild-type plants, the expression of the ABA and abiotic stress-responsive genes, ABI1, ABI2, KAT1, KAT2, DREB2A, RD29A, and RD29B, was decreased in axe1-5 and HDA6 RNA-interfering plants when treated with ABA or salt stress. It was found that both ABA and salt stress could enrich the gene activation markers, histone H3K9K14 acetylation, and H3K4 trimethylation, but decrease the gene repression marker, H3K9 dimethylation, of the ABA and abiotic stress-responsive genes. Our study indicates that HDA6-involved histone modifications modulate seed germination and the salt stress response, as well as ABA- and salt stress-induced gene expression in Arabidopsis.