Myeloperoxidase expression in diencephalon is potentially associated with fear‐related behavior in chicks of laying hen
Myeloperoxidase expression in diencephalon is potentially associated with fear‐related behavior in chicks of laying hen
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间脑中的髓过氧化物酶表达可能与蛋鸡雏鸡的恐惧相关行为有关
DOI：
10.1111/asj.13779
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发表时间：
2022
影响因子：
2
通讯作者：
Tokutake Yukako
中科院分区：
文献类型：
--
作者：
Kohno Suzuka;Ogawa Shinichiro;Shimmura Tsuyoshi;Sato Kan;Tokutake Yukako
预防成年蛋鸡啄毛现象对集约化家禽养殖具有重要意义。生长中的母鸡蛋鸡的恐惧相关行为可能预测成年母鸡的生育能力。在这项研究中，我们在两个具有不同FP频率的代表性产蛋品种（White Leghorn [WL]和Rhode Island Red [RIR]）中发现了一个与小鸡恐惧相关行为和成年母鸡FP相关的候选基因。在强直不动试验和空地试验中，白岭鸡的行为活动低于RIR鸡（P< 0.01），表明白岭鸡比RIR鸡更可怕。在前人研究的基础上，我们选择了51个在高FP和低FP群体大脑中表达差异的基因，并对它们在鸡间脑中的表达水平进行了筛选。结果表明，骨髓过氧化物酶（MPO）基因在WL鸡中的表达量高于RIR鸡（P< 0.05）。此外，STRING分析预测了包括mpo和mpo相关基因在内的基因网络，并揭示了这些基因与恐惧相关行为的关联。这些结果表明，mpois可能与生长中的母鸡蛋鸡的恐惧相关行为和成年母鸡的FP有关。
Preventing feather pecking (FP) in adult laying hens is important for the welfare of intensively poultry farming. Fear‐related behavior in growing female layer chicks may predict FP in adult hens. In this study, in two representative laying breeds (White Leghorn [WL] and Rhode Island Red [RIR]) that have different FP frequencies, we identified a candidate gene associated with fear‐related behavior in chicks and FP in adult hens. In the tonic immobility test and open‐field test, the behavioral activity was lower in WL chicks than in RIR chicks (P< 0.01), suggesting that WL chicks were more fearful than RIR chicks. Based on previous studies, 51 genes that have been found to be differentially expressed in the brain between high‐ and low‐FP populations were chosen, and their expression levels were screened in the chick diencephalon. This analysis revealed that myeloperoxidase (MPO) gene expression level was higher in WL chicks than that in RIR chicks (P< 0.05). Furthermore, STRING analysis predicted the gene network includingMPOandMPO‐related genes and revealed the association of these genes with fear‐related behavior. These results suggest thatMPOis potentially associated with fear‐related behavior in growing female layer chicks and FP in adult hens.