Preclinical Assessment of a Tissue-Engineered Vasomotive Human Small-Calibered Vessel Based on a Decellularized Xenogenic Matrix: Histological and Functional Characterization
Preclinical Assessment of a Tissue-Engineered Vasomotive Human Small-Calibered Vessel Based on a Decellularized Xenogenic Matrix: Histological and Functional Characterization
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DOI：
10.1089/ten.tea.2010.0375
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发表时间：
2011-05-01
影响因子：
4.1
通讯作者：
Kallenbach, Klaus
中科院分区：
文献类型：
--
作者：
Heine, Joerg;Schmiedl, Andreas;Kallenbach, Klaus
目的：组织工程动脉血管（TEAV）在小口径人体旁路移植术和血管化支架应用中具有实质性的优势。然而，TEAV的组织学组成必须考虑功能特性，如血管舒缩性。本研究的目的是表征人类teav的形态和血管舒缩性。方法：选取直径< 5mm的猪颈动脉段（天然[NA， n = 6]，脱细胞[DA， n = 6]，生物反应器脱细胞/再植入人血管内皮细胞[hvECs]和平滑肌细胞[hvSMCs]） 3组进行检测。采用光镜和扫描电镜，并在krebs溶液中进行hvSMCs和hvecs相关的血管舒张性试验，对活化teav进行表征。结果：DAs形态学检查显示无细胞外基质。光镜下在DAs的横截面上显示完整的细胞外基质成分呈圆形层状形成。RAs显示，小细胞沿着管腔部位剩余的内侧纤维结构和扁平细胞层迁移，被鉴定为hvECs和hvSMCs，其CD-31和α -肌动蛋白信号传导比对照组低。扫描电镜显示RAs的管腔表面有完整的扁平细胞层，介质部位有致密的hvSMCs。DAs刺激后应变减小。与DAs相比，RAs恢复了血管舒张力，但与NAs相比，RAs表现出收缩减少和不完全松弛。结论：本研究表明，人血管细胞活化DA与na样形态相似，可以保证teav的血管舒缩性。
Objectives: Tissue-engineered arterial vessels (TEAV) offer substantial advantages in small-calibered human-bypass- grafting and vascularized scaffold applications. However, histological composition of TEAV must allow for functional properties, such as vasomotoricity. Aim of this study was to characterize human TEAVs regarding morphology and vasomotoricity.Methods: Three groups containing segments of porcine carotid artery < 5mm in diameter (native [NA, n = 6], decellularized [DA, n = 6], and decellularized/reseeded in a bioreactor [RA, n 7] with human vascular endothelial [hvECs] and smooth muscle cells [hvSMCs]) were examined. Light and scanning electron microscopy were applied, and hvSMCs- and hvECs-associated Vasomotoricity Test conducted in Krebs-solution was used for characterization of revitalized TEAVs.Results: Morphologic examination showed cell-free extracellular matrix in DAs. Light microscopy demonstrated intact extracellular matrix components in circle-layered formation in cross sections of DAs. RAs showed small cells migrating along the remaining medial fiber structures and flat cell layers at the luminal site, identified as hvECs and hvSMCs with lower CD-31 and alpha-actin signaling than controls. Scanning electron microscopy showed intact flat cell layers on luminal surfaces of RAs and dense hvSMCs at their media site. DAs showed decreasing strain after stimulation. RAs retrieved vasomotoricity compared to DAs, but showed reduced contraction and incomplete relaxation compared to NAs.Conclusions: This study shows that revitalization of DA with human vascular cells resembles NA-like morphology and can ensure vasomotoricity of TEAVs.