Pseudomonas corrugata crpCDE is part of the cyclic lipopeptide corpeptin biosynthetic gene cluster and is involved in bacterial virulence in tomato and in hypersensitive response in Nicotiana benthamiana.
Pseudomonas corrugata crpCDE is part of the cyclic lipopeptide corpeptin biosynthetic gene cluster and is involved in bacterial virulence in tomato and in hypersensitive response in Nicotiana benthamiana.
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DOI：
10.1111/mpp.12207
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发表时间：
2015-06
影响因子：
4.9
通讯作者：
C. Strano;P. Bella;G. Licciardello;A. Fiore;A. R. Lo Piero;V. Fogliano;V. Venturi;V. Catara
中科院分区：
文献类型：
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作者：
C. Strano;P. Bella;G. Licciardello;A. Fiore;A. R. Lo Piero;V. Fogliano;V. Venturi;V. Catara
皱皮假单胞菌CFBP 5454产生两种环脂肽（CLPs），分别是考霉素A和考肽素，这两种环脂肽都具有表面活性、抑菌和植物毒性。在这项研究中，我们确定了编码一个假定的非核糖体肽合成酶和ABC型运输系统参与corpeptin生产的基因。这些基因属于相同的转录单位，命名为crpCDE。该位点的遗传组织与其他假单胞菌CLP生物合成簇高度相似。基质辅助激光解吸电离飞行时间质谱（MALDI-TOF-MS）分析显示，转运蛋白和合成酶基因组敲除突变体不能产生corpeptin，但继续产生cormycin A。这表明CrpCDE是参与皱皮侧耳CFBP 5454中抑肽酶产生的唯一系统。此外，系统发育分析显示，CrpE ABC转运蛋白与CLPs的转运蛋白聚簇，具有长肽链。在corpeptin生产耗尽菌株的毒性显着低于野生型菌株接种时，番茄植株和只诱导萎黄病时，渗入本氏烟草叶。因此，corpeptin是皱叶侧耳与植物相互作用的重要效应因子。表达分析表明，crpC转录发生在高细胞密度。两种LuxR转录调节因子PcoR和RfiA在crpC表达中具有关键作用，从而在corpeptin产生中具有关键作用。
Pseudomonas corrugata CFBP 5454 produces two kinds of cyclic lipopeptides (CLPs), cormycin A and corpeptins, both of which possess surfactant, antimicrobial and phytotoxic activities. In this study, we identified genes coding for a putative non-ribosomal peptide synthetase and an ABC-type transport system involved in corpeptin production. These genes belong to the same transcriptional unit, designated crpCDE. The genetic organization of this locus is highly similar to other Pseudomonas CLP biosynthetic clusters. Matrix-assisted laser desorption ionization-time of flight-mass spectrometry (MALDI-TOF-MS) analysis revealed that transporter and synthetase genomic knock-out mutants were unable to produce corpeptins, but continued to produce cormycin A. This suggests that CrpCDE is the only system involved in corpeptin production in P. corrugata CFBP 5454. In addition, phylogenetic analysis revealed that the CrpE ABC transporter clustered with the transporters of CLPs with a long peptide chain. Strains depleted in corpeptin production were significantly less virulent than the wild-type strain when inoculated in tomato plants and induced only chlorosis when infiltrated into Nicotiana benthamiana leaves. Thus, corpeptins are important effectors of P. corrugata interaction with plants. Expression analysis revealed that crpC transcription occurs at high cell density. Two LuxR transcriptional regulators, PcoR and RfiA, have a pivotal role in crpC expression and thus in corpeptin production.