Transcriptome reveals overview of Ca2+ dose-dependent metabolism disorders in zebrafish larvae after Cd2+ exposure.
Transcriptome reveals overview of Ca2+ dose-dependent metabolism disorders in zebrafish larvae after Cd2+ exposure.
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DOI：
10.1016/j.jes.2021.12.009
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发表时间：
2021-12
影响因子：
6.9
通讯作者：
B. Zhu;Ziniu Wang;Lei Lei-Lei;Yongyong Guo;Jian Han;Bingsheng Zhou
中科院分区：
文献类型：
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作者：
B. Zhu;Ziniu Wang;Lei Lei-Lei;Yongyong Guo;Jian Han;Bingsheng Zhou
镉（Cd）是一种普遍存在的环境有害重金属，对包括硬骨鱼在内的水生生物的健康构成重大威胁。虽然镉的毒性分布已得到充分认识，但在个体水平上对不同水生环境参数（例如水硬度）的毒性反应的总体看法知之甚少。在这里，通过调节E3介质中的Ca2+水平部分模拟了水硬度的差异。作为anin体内模型，斑马鱼胚胎暴露于不同的Ca2+水平（NV，正常Ca2+； LV，低Ca2+； HV，高Ca2+）单独或联合30.7µg/L Cd2+（分别为NC， LC和HC），直到受精后144小时。全基因组转录组揭示了组间基因表达的差异。功能富集分析发现，与代谢相关的生物过程，特别是脂质代谢，在NC和LC处理中明显中断，而在HC组中观察到缓解。生化分析证实，Ca2+的减少增强了Cd2+处理下的合成，抑制了动员，增加了脂质的储存。本研究提示Ca2+会影响Cd对生物通路的毒性作用，这将提高对Cd的毒理学认识，有助于对Cd的准确评估。
Cadmium (Cd), a ubiquitous environmental hazardous heavy metal, poses a significant threat to the health of aquatic organisms, including teleosts. Although the toxic profile of Cd is well recognized, little is known regarding the overall view of toxic responses to varying aquatic environmental parameters (e.g., water hardness) at an individual level. Herein, differences in water hardness were partially mimicked by adjusting Ca2+levels in E3 medium. As anin vivomodel, zebrafish embryos were exposed to variable Ca2+levels (NV, normal Ca2+; LV, low Ca2+; HV, high Ca2+) alone or combined with 30.7 µg/L Cd2+(NC, LC, and HC, respectively) until 144 hr post-fertilization. The genome-wide transcriptome revealed differentially expressed genes between groups. Functional enrichment analysis found that biological processes related to metabolism, particularly lipid metabolism, were significantly disrupted in NC and LC treatments, while a remission was observed in the HC group. Biochemical assays confirmed that the decrease in Ca2+enhanced synthesis, inhibited mobilization and increased the storage of lipids in Cd2+treatments. This study suggests that the toxic effect of Cd on biological pathways will be influenced by Ca2+, which will improve the toxicological understanding and facilitate accurate assessment of Cd.