Structure-function evolution of the Transforming acidic coiled coil genes revealed by analysis of phylogenetically diverse organisms
Structure-function evolution of the Transforming acidic coiled coil genes revealed by analysis of phylogenetically diverse organisms
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DOI：
10.1186/1471-2148-4-16
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发表时间：
2004-06-18
影响因子：
3.4
通讯作者：
Liang, P
中科院分区：
文献类型：
--
作者：
Still, IH;Vettaikkorumakankauv, AK;Liang, P
背景：对古老基因家族的研究可以提供一个深入了解基因结构的进化如何与功能相关的视角。进化上保守的转化酸性卷曲螺旋（TACC）基因家族的功能同源物存在于从酵母到man. However，这些蛋白质的功能相互作用和进化之间的相关性尚未被determined.Results：我们已经进行了广泛的数据库分析，以确定基因组和cDNA序列的TACCs从遗传多样性的生物。该分析已经确定了TACC蛋白与其他卷曲螺旋蛋白的系统发育关系，兔TACC 4作为人TACC 3的直系同源物的位置的分辨率，以及RHAMM作为卷曲螺旋蛋白的不同家族。我们还将TACCs的分析扩展到C. elegans和D.黑腹动物，以确定潜在的新的TACC相互作用。该模型的有效性被证实独立的人TACC 2的核激素受体RXR β.Conclusion的直接结合的演示：迄今为止的数据表明，祖先TACC蛋白质发挥了作用，在中心体/有丝分裂纺锤体动力学。然后，通过与特定桥接蛋白的相互作用，将TACC蛋白募集到参与蛋白质翻译、RNA加工和转录的复合物中。然而，在进化过程中，TACC蛋白现在已经获得了与这些复合物的组分（如LSm蛋白、核激素受体、GAS 41和转录因子）直接相互作用的能力。这表明，TACC蛋白的功能可能已经从中心体中执行组装或协调功能演变为在细胞中染色质重塑、转录和转录后复合物的功能进化中发挥更密切的作用。
Background: Examination of ancient gene families can provide an insight into how the evolution of gene structure can relate to function. Functional homologs of the evolutionarily conserved transforming acidic coiled coil (TACC) gene family are present in organisms from yeast to man. However, correlations between functional interactions and the evolution of these proteins have yet to be determined.Results: We have performed an extensive database analysis to determine the genomic and cDNA sequences of the TACCs from phylogenetically diverse organisms. This analysis has determined the phylogenetic relationship of the TACC proteins to other coiled coil proteins, the resolution of the placement of the rabbit TACC4 as the orthologue of human TACC3, and RHAMM as a distinct family of coiled coil proteins. We have also extended the analysis of the TACCs to the interaction databases of C. elegans and D. melanogaster to identify potentially novel TACC interactions. The validity of this modeling was confirmed independently by the demonstration of direct binding of human TACC2 to the nuclear hormone receptor RXRbeta.Conclusion: The data so far suggest that the ancestral TACC protein played a role in centrosomal/mitotic spindle dynamics. TACC proteins were then recruited to complexes involved in protein translation, RNA processing and transcription by interactions with specific bridging proteins. However, during evolution, the TACC proteins have now acquired the ability to directly interact with components of these complexes (such as the LSm proteins, nuclear hormone receptors, GAS41, and transcription factors). This suggests that the function of the TACC proteins may have evolved from performing assembly or coordination functions in the centrosome to include a more intimate role in the functional evolution of chromatin remodeling, transcriptional and posttranscriptional complexes in the cell.