Apolipoprotein A-I-containing lipoproteins with pre-beta electrophoretic mobility.
Apolipoprotein A-I-containing lipoproteins with pre-beta electrophoretic mobility.
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发表时间：
1985-05
影响因子：
6.5
通讯作者：
S. Kunitake;Kathleen J. La;J. Kane
中科院分区：
文献类型：
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作者：
S. Kunitake;Kathleen J. La;J. Kane
在通过选择性亲和免疫吸附从人血清和血浆中分离出的含 apoA-I 的脂蛋白中，我们鉴定了一个亚群，该亚群与大部分高密度脂蛋白不同，具有前 β 电泳迁移率。该前β亚群可以通过免疫电泳直接在新鲜血浆中观察到。它含有磷脂、游离胆固醇和酯化胆固醇，但蛋白质占其质量的 90%。载脂蛋白 A-I 是该亚群中主要的载脂蛋白；未检测到载脂蛋白 A-II 和 B 脂蛋白。该亚群的蛋白质部分表现出明显低于通过超速离心分离的高密度脂蛋白的螺旋度。
Among the apoA-I-containing lipoproteins isolated by selected-affinity immunosorption from human serum and plasma, we have identified a subpopulation which, unlike the bulk of high density lipoproteins, has pre-beta electrophoretic mobility. This pre-beta subpopulation can be observed directly in fresh plasma by immunoelectrophoresis. It contains phospholipid and free and esterified cholesterol, but protein constitutes 90% of its mass. Apolipoprotein A-I is the predominant apolipoprotein in this subpopulation; apolipoprotein A-II and the B lipoproteins are not detected. The protein moiety of this subpopulation exhibits markedly lower helicity than that of high density lipoproteins isolated by ultracentrifugation.