Immune signaling pathways regulating bacterial and malaria parasite infection of the mosquito Anopheles gambiae
Immune signaling pathways regulating bacterial and malaria parasite infection of the mosquito Anopheles gambiae
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DOI：
10.1073/pnas.0504950102
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发表时间：
2005-08-09
影响因子：
11.1
通讯作者：
Zheng, LB
中科院分区：
文献类型：
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作者：
Meister, S;Kanzok, SM;Zheng, LB
我们发现，在疟疾媒介冈比亚按蚊中，天蚕素1的表达受REL2调控，REL2是一种与果蝇口味同源的核因子-kappa B类转录因子。通过选择性剪接，REL2产生一个全长的(REL2-F)和一个较短的(REL2-S)蛋白亚型，缺少抑制性连接蛋白重复序列和死亡结构域。RNA干扰实验表明，与只对革兰氏阴性菌起反应的果蝇不同，按蚊的REL2-F和REL2-S亚型分别参与防御革兰氏阳性金黄色葡萄球菌和革兰氏阴性大肠埃希菌。REL2-F还调节蚊子感染疟疾寄生虫伯氏疟原虫的强度。适配器IMD与REL2-F具有相同的活动。微阵列分析确定了另外10个受REL2调控的基因，包括CEC3、GAM1和LRIM1。
We show that, in the malaria vector Anopheles gambiae, expression of Cecropin 1 is regulated by REL2, an NF-kappa B-like transcription factor orthologous to Drosophila Relish. Through alternative splicing, REL2 produces a full-length (REL2-F) and a shorter (REL2-S) protein isoform lacking the inhibitory ankyrin repeats and death domain. RNA interference experiments show that, in contrast to Drosophila Relish, which responds solely to Gram-negative bacteria, the Anopheles REL2-F and REL2-S isoforms are involved in defense against the Gram-positive Staphylococcus aureus and the Gram-negative Escherichia coli bacteria, respectively. REL2-F also regulates the intensity of mosquito infection with the malaria parasite, Plasmodium berghei. The adaptor IMD shares the same activities as REL2-F. Microarray analysis identified 10 additional genes regulated by REL2, including CEC3, GAM1, and LRIM1.