Target delivery of a gene into the brain using the RVG29-oligoarginine peptide
Target delivery of a gene into the brain using the RVG29-oligoarginine peptide
复制标题
使用 RVG29-寡精氨酸肽将基因靶向递送至大脑
DOI：
10.1016/j.biomaterials.2011.12.017
复制
发表时间：
2012-04-01
期刊：
影响因子：
14
通讯作者：
Zhang, Yu-Hui
中科院分区：
文献类型：
--
作者：
Gong, Cheng;Li, Xiangning;Zhang, Yu-Hui
开发能够介导DNA通过血脑屏障进入大脑的高效、排他性和非侵入性转移的非病毒传递系统具有挑战性，但对于基因治疗脑疾病的临床应用至关重要。与其他非病毒DNA载体（如脂质或聚合物）相比，基于肽的DNA传递系统具有许多优点，包括易于合成、低免疫原性、生物相容性和体内生物降解性。然而，所有现有的基于肽的DNA递送载体都缺乏对细胞或组织的选择性，这在很大程度上限制了它们在体内的应用。在这项研究中，我们证明了基于RVG29-9rR肽的DNA递送系统能够比Lipofectamine LTX & Plus（最有效的市售转染试剂之一）更有效和特异性地在体外转染Neuro 2a细胞。更重要的是，经小鼠静脉注射后，该肽介导了高效的脑靶向报告基因表达。因此，本文的结果提示了一种新的脑靶向DNA体内递送策略。(C) 2012 Elsevier Ltd.版权所有。
The development of non-viral delivery systems that are capable of mediating an efficient, exclusive, and non-invasive transfer of DNA across the blood brain barrier into the brain is challenging, but essential for the clinical application of gene therapy to brain diseases. Compared with other non-viral DNA carriers (e.g., lipids or polymers), peptide-based DNA delivery systems have many advantages including the ease of synthesis, low immunogenicity, biocompatibility, and biodegradability in vivo. However, all of the existing peptide-based vehicles for DNA delivery lack selectivity toward cells or tissues, which largely limited their applications in vivo. In this study, we demonstrated that an RVG29-9rR peptide-based DNA delivery system was able to transfect Neuro 2a cells in vitro more efficiently and specifically than Lipofectamine LTX & Plus, one of the most efficient commercially available transfection reagents. More significantly, the peptide mediated efficient and brain-targeting reporter gene expression after intravenous injection into mice. Thus, the results herein suggest a new strategy for brain-targeting DNA delivery in vivo. (C) 2012 Elsevier Ltd. All rights reserved.